Slide scanning submission form

Name *

Email address *

PI *

University or Company

Speed type

Sample type Slides 96 well plate 24 well plate 48 well plate 6 well plate

Number of slides or plates to be imaged

Multi-well plate: number of wells to image

Multi-well plate: Which wells will be imaged? (example: A1-B12)

My samples are tissue sections cultured cells other

Area to be imaged Entire tissue section/coverslip/well area Partial sample (to be specified below)

If partial sample area is to be imaged, please specify number of fields of view requested per sample:

Select scan type * Color (H&E) Fluorescence (Widefield - available for slides and multi-well plates) Fluorescence (Confocal - only available for slides)

If fluorescence, please list all stains (ie DAPI, Cy3, AF488, etc) in your samples.

If fluorescence, which slide or well is your positive control? Which slide or well is your negative control?

If fluorescence, what is your brightest sample (used to set appropriate settings)? *

Select magnification below. Widefield 20x magnification is recommended for the majority of samples. If magnification at 40x desired, please include a description of the sample and the need for higher magnification images in the notes section of this form. If you would like magnification above 40x, please contact us for options. 10x (lowest magnification and resolution) 20x (most common and sufficient for most experiments) 40x (only available for fluorescent samples)

Widefield color camera (SL only) , fluorescence (SL and iQ) or Confocal (iQ only)? Please note that the iQ confocal scanner does NOT have a camera for slide labels. Slides will be labeled numerically by the SCOPE in the order submitted unless users submit a spreadsheet with label information to the SCOPE. Widefield color imaging (for H&E and similar stains, SL only) Widefield fluorescence (most common, SL and iQ scanners) Confocal (highest resolution, only available for fluorescent samples, iQ only) Not sure

If you will be comparing these images to scans previously acquired in the SCOPE, please enter the date and file name of the previous scans. That will allow us to use the same image settings.

Please store my samples at: * room temperature 4 degrees -20 degrees

Please enter a short summary of your samples and the expected signal from each stain below. If confocal, extended z-stacks or high magnification scans are requested, please describe that need below. The SCOPE will contact you to discuss your experimental requirements in more detail if necessary. *

I would like my data transferred Through Dropbox Through WeTransfer Another method (contact SCOPE@umassmed.edu)

Enter the date you plan to drop off samples. *

FOR EXTERNAL USERS ONLY: By clicking this button, I confirm that I have an approved Biosafety Form on file with the SCOPE for this sample type. The Biosafety Form can be downloaded from the Forms and Resources page on the SCOPE website (https://www.umassmed.edu/scope/forms-and-resources/) Yes

The SCOPE images samples as received. We will work with users to obtain the best images possible given the submitted sample quality. Dirt, fibers, air bubbles, non-flat tissue, and other sample quality issues will result in poor images. By using the SCOPE imaging service, users agree to pay all imaging fees, regardless of resulting image quality. * I understand that my image quality depends upon my samples and I agree to pay the SCOPE imaging fees for this service.

Slide scanning sample submission form