Nikon A-1 Confocal/Wide Field Scope
This instrument utilizes the Nikon Ti scope base and Nikon Elements AR software for scope control and image capture and quantification. All subsystems are fully automated providing for:
Time lapse imaging
Multi-point revisiting
Multi-channel fluorescence plus DIC imaging (volume view)
Z stack imaging with 3D reconstruction
Automated tiling/stitching for large specimin imaging at high magnification
Movie making from time lapse images
DIC optics are available on all of the following objectives except the 2X
Magnification | Lens Type | N.Aperture |
2X | Plan Apo | 0.1 |
10X | Plan Fluor | 0.3 |
20X | Plan Fluor | 0.5 |
40X | Plan Fluor | 1.3 |
60X | Apo TIRF | 1.49 |
100X | Plan Apo | 1.40 |
3 independant imaging systems are available:
- Nikon A-1 confocal system providing 7 available laser lines:
405, 457, 476, 488, 514, 561, 640 and 12 bit capture - Photometrics QuantEM high sensitivity monochrome widefield fluorescence camera with 16 bit capture.
- Nikon DS-i2 widefield color camera with 16 bit capture
Filter Cubes (eyepiece viewing and widefield imaging)
Name Excitation Emission
DAPI 375-400 435-486
GFP 450-495 500-550
TXRed 540-580 593-668
CY5 590-650 665-745