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General Sorting Information

Machine

Aria II/IIu
(BSL-2 and BSL-2+)

Sony SH800 
BDL-2+

BSL-3 FACS Aria IIu

Lasers
Parameters

 (BL2) 5B 3V 3R 2UV 5YG 

"C" Aria


(BL2) 5B 2V 2R 

"S" Aria


 (BL2+) 2B 6V 3R 5YG 

"2" Aria

488/633/405/561
(co-linear lasers)

5B 3V 4R 2UV 

Nozzle sizes (µm) 70/85/100/130
70/100 70/85/100
Cell Concentration 1-2 x107 1-2 x107 1-2 x107
Sort Rate  up to 25,000 cells/sec. up to 5,000 cells/sec. up to 25,000 cells/sec.
Operating Pressure (psi) 70/45/20/10
20/40 70/45/20

Running media - 1% FBS in any phenol-red-free media. HEPES can be added at a final concentration of 25mM to media to keep cells well buffered for all-day sorts, and/or to collection media.

Collection media - this is usually 20% FBS in whichever media the cells are happiest. This is diluted 4-5x and ends up being approximately 5% FBS.

Collection plates: bring plates for collection with media pre-loaded in the wells. Do not fill wells above 1/2 maximum capacity to avoid spilling.

Filtering : All samples are filtered through 37µ mesh prior to running by core facility personel, however if you wish to filter the samples yourself refer to the ordering information below.

Sterile 12x75 mm tubes with filter caps are available; by Corning - Fisher Cat. number 08-771-23

Nitex Nylon Filtration Mesh order from: ELKO Filtering Co, LLC. (elkofiltering.com) Nylon mesh roll 37µ opening size, 40" wide, sold by the yard) Item#: 03-37/24 

Miltenyi MACS® SmartStrainers  #130-098-458

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