Biosafety at the Flow Cytometry Core Lab
Cell sorting experiments which include human or non-human primate cells or cell lines in the BSL2 Core lab must supply either the screening information as listed below or have documentation from the source of the cells such as ATCC. If you cannot provide this information the samples will have to be sorted in our BSL3 facility.
These policies are in accordance with our Environmental Health and Safety specifications and are for the safety of the staff and any investigators who may be in the facility.
All unfixed human or non-human primate samples submitted to the Flow Cytometry Core for analysis must be scheduled in advance identifying the source of the sample as well as the potential biohazard. Human or non-human primate samples which have screening information need to submit the documentation along with your data sheet. Screening information includes:
- Hepatitis A, B and C
- HIV 1 and 2
- HTLV 1 and 2
- West Nile Virus
If unfixed human or primate samples are brought to the Core lab without advance scheduling or the required paperwork they will not be run. Our Sort Sample Request Form must be filled out and presented with BSL2+ and BSL3 sorts. IBC Docket numbers are required on all Sample Request Forms and a Flow Sorting Addendum must be filed with your IBC protocol. This addendum can be found at the IBC website.
Further information can be found at the ISAC website: http://isac-net.org/Resources-for-Cytometrists/Biosafety.aspx