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Publications 2020-2016

Duan Y, Choi S, Nelson C, Ambros V.   Engineering essential genes with a "jump board" strategy using CRISPR/Cas9. MicroPubl Biol. 2020 Oct 8;2020. doi: 10.17912/micropub.biology.000315. PMID: 33274316.  PMCID: PMC7704125.  (Paper)

Vasquez-Rifo A, Ricci, EP, Ambros, V.   Pseudomonas aeruginosa cleaves the decoding center of Caenorhabditis elegans ribosomes.   PLoS Biol 2020 Dec 1;18(12): doi: 10.1371/journal.pbio.3000969. eCollection 2020 Dec. PMID: 33259473.  (Paper)

Duan, Y., Yongming Sun, Y., Ambros. V. RNA-seq with RNase H-based ribosomal RNA depletion specifically designed for C. elegans. MicroPubl Biol. 2020 Sep 22;2020:10.17912/micropub.biology.000312. doi: 10.17912/micropub.biology.000312. PMID: 33005886. PMCID: PMC7520128. (Paper)

Geekiyanage, H., Rayatpisheh, S., Wohlschlegel, J.A., Brown, Robert, Jr., Ambros, V. Extracellular microRNAs in human circulation are associated with miRISC complexes that are accessible to anti-AGO2 antibody and can bind target mimic oligonucleotides. Proc Natl Acad Sci U S A . 2020 Sep 29;117(39):24213-24223. doi: 10.1073/pnas.2008323117. Epub 2020 Sep 14. PMID: 32929008. PMCID: PMC7533700.  (Paper)

Carreiro, S, James Marvel-Coen, J., Lee, R., Chapman, B., Ambros, V. Circulating microRNA Profiles in Acetaminophen Toxicity. J Med Toxicol. 2020 Apr;16(2):177-187. doi: 10.1007/s13181-019-00739-6. Epub 2019 Dec 2. PMID: 31792846. PMCID: PMC7099119 (available on 2021-04-01). (Paper)

Ilbay, O, Ambros V.   Regulation of nuclear-cytoplasmic partitioning by the lin-28-lin-46 pathway reinforces microRNA repression of HBL-1 to confer robust cell-fate progression in C. elegans. Development. 2019 Nov 6;146(21). pii: dev183111. doi: 10.1242/dev.183111. PMID: 31597658. PMCID: PMC6857590. (Paper)

Nelson C, Ambros V.   Trans-splicing of the C. elegans let-7 primary transcript developmentally regulates let-7 microRNA biogenesis and let-7 family microRNA activity. Development. 2019 July 24;146(14). pii: dev182212. doi: 10.1242/dev.182212. PMID: 31340934. PMCID: PMC6432665. (Paper)

Ilbay, O, Ambros V.   Pheromones and Nutritional Signals Regulate the Developmental Reliance on let-7 Family MicroRNAs in C. elegans. Curr Biol. 2019 Jun 3;29(11):1735-1745.e4. doi: 10.1016/j.cub.2019.04.034. Epub 2019 May 16. PMID: 31104929. (Paper)

Choi S, Ambros V. The C. elegans heterochronic gene lin-28 coordinates the timing of hypodermal and somatic gonadal programs for hermaphrodite reproductive system morphogenesis. Development. 2019 Feb 11. pii: dev.164293. doi: 10.1242/dev.164293.PMID: 30745431. PMCID: PMC6432661. (Paper)

Vasquez-Rifo, A., Veksler-Lublinsky, I., Cheng, Z., Ausubel, F.M., Ambros, V. The Pseudomonas aeruginosa accessory genome elements influence virulence towards Caenorhabditis elegans. Genome Biol. 2019; 20:270. doi:  PMID: 31823826. PMCID: PMC6902481. (Paper)

Ambros, V, Ruvkun, G. Recent Molecular Genetic Explorations of Caenorhabditis elegans MicroRNAs.   Genetics. 2018 Jul;209(3):651-673. doi: 10.1534/genetics.118.300291.   PMID:29967059. PMCID: PMC6028246. (Paper)

McJunkin K, Ambros V. A microRNA family exerts maternal control on sex determination in C. elegans. Genes Dev. 2017 Feb 15;31(4):422-437. doi: 10.1101/gad.290155.116. Epub 2017 Mar 9. PMID: 28279983. PMCID:PMC5358761. (Paper)

Tanriverdi K, Kucukural A, Mikhalev E, Tanriverdi SE, Lee R, Ambros VR, Freedman JE. Comparison of RNA isolation and associated methods for extracellular RNA detection by high-throughput quantitative polymerase chain reaction. Anal Biochem. 2016 May 15;501:66-74. doi: 10.1016/j.ab.2016.02.019. Epub 2016 Mar 10. PMID:26969789. (Paper)

Ren, Z, Veksler-Lublinsky I, Morrissey D, Ambros V. Staufen negatively modulates microRNA activity in Caenorhabditis elegans. G3 (Bethesda). 2016 May 3;6(5):1227-37. doi: 10.1534/g3.116.027300. PMID:26921297.   PMCID: PMC4856075.  (Paper)