Mammalian erythropoiesis is the process by which blood stem cells differentiate into red blood cells. It is an accessible experimental system that allows us to address fundamental questions in biology and disease. Erythropoietic deficits are common worldwide, manifesting as anemia or malignant conditions such as myelodysplasia or myeloproliferative disease.
We investigate how cell fate decisions during erythropoiesis are orchestrated by DNA replication and by epigenetic mechanisms. Using single-cell RNA-seq, we recently outlined the entire trajectory of erythroid development, starting with the hematopoietic stem cell, and identifying new erythropoietic growth factor regulators. We also study the systems biology principles that allow a massive ramping up of red cell production in response to the stress of bleeding, anemia or hypoxia.
Population Snapshots Predict Early Hematopoietic and Erythroid Hierarchies. Khoramian Tusi et al., Nature, 2018. doi:10.1038/nature25741 [view in http://rdcu.be/HxCF]
High Throughput Single-Cell Fate Potential Assay of Murine Hematopoietic Progenitors in Vitro. Khoramian Tusi et al., Experimental Hematology, 2018. doi: 10.1016/j.exphem.2018.01.005
Global Increase in Replication Fork Speed During a p57KIP2-Regulated Erythroid Cell Fate Switch. Hwang et al., Science Advances, 2017 [View in: Science Advances]