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Cryo-EM Basics

What is Cryo-EM? 

Cryo-EM - Cryo-Electron Microscopy is a technique used in structural biology. It uses high speed electrons as source beam to obtain structural information of the biological system studied. Usually, the biological sample is embedded in vitreous ice by rapid freezing. The cryo sample is then observed and imaged in an electron microscope under liquid nitrogen temperature. 

If you are new to Cryo-EM, you might find this short youtube video made by Gabriel Lander useful.

Cryo-EM Specimen Preparation Pipeline 

Below are two pipelines for cryo-EM specimen preparation for both Single Particle and Electron Tomography applications.

Single Particle

cryo grid prep pipeline single particle


cryo grid prep pipeline tomo