PBCE Services
Library Prep and Sequencing
- Our package deal includes library building and sequencing of one SMRTcell.
- Additional SMRTcells can be added at a discounted price.
- Size-selection of large fragments on the Sage Science Blue Pippin is available separately or bundled as part of certain library prep packages.
- Multiplexed library builds are available. The options for this are:
barcodes internal to your amplicon(s) (you would have to add these during amplification using PacBio's Barcoded Universal Primers) or barcodes in the PacBio SMRTbell adapters (added by the Core during library prep). - Material may be turned in as RNA or DNA. If you are off-campus, PacBio has some recommended guidelines for sample shipping.
Bioinformatics
- Bioinformatics services offered include:
Consensus/CCS (ROI/Read Of Insert) analysis, IsoSeq analysis, assembly, mapping, variant, motif, and/or base modification detection options, and demultiplexing. - Data delivery options for non-UMass customers are to have the data uploaded to Globus. (You do not need a full Globus subscription to receive data, however you do need a registered email account. Information about Globus subscription and personal use is available at https://app.globus.org.)
| Library Types: |
|---|
| Amplicon or short fragment |
| AAV fragments (range of sizes, possibly single-stranded) |
| IsoSeq / cDNA (from total RNA) |
| Genomic or plasmid DNA (includes shearing step) |
| HiFi Genomic (tightly sized for <2Gb genomes) |
| optional add-on: Indexed (for multiple lib. types) |
| Imaging Times: |
|---|
| 10 hours (recommended for short amplicons and 16S) |
| 15 hours (microbial de novo and structural variants) |
| 20 hours (by special request) |
| 24 hours (AAV and IsoSeq libraries) |
| 30 hours (HiFi, metagenomics, de novo low input) |
Pricing and Payment Policy
Please email PacBio@umassmed.edu to get a quote for your desired services.
Processing and analyzing a sample requires time and reagents. Payment for these services is the responsibility of the user submitting the sample and should be rendered in a timely fashion. In the event of a reagent or equipment failure, the samples will be rerun at the next possible opportunity at no additional charge.
Clients withdrawing samples prior to the analysis run will be charged a fee to recover QC assay costs. For the return of archived post-analysis samples, clients will be charged a delivery fee per sample.
A signed submission ticket or email authorization indicates that the holder of the account to be charged consents to payment in accordance with this policy.
Inclement Weather Policy
In case of extreme and/or inconvenient weather, the machines will still be running but we may not be here for sample login, pickup, or delivery. Please email us to arrange drop-off if the weather is questionable. The PBCE, DSCL, & MBCL are closed when UMass Chan is on "inclement weather alert".