Deep Sequencing Core Labs
The Deep Sequencing Core Facility offers high-throughput sequencing and library-building services for the UMass campuses and outside customers.
Our instruments include the Illumina HiSeq 4000, HiSeq 2000, and MiSeq. Check out the Services page for detailed run options.
For a sequencing pricing list, details on specific services, scheduling, or a quote for services, please email us here.
The Core has 3 dropoff locations where samples can be left in the
Biotech2 outside Suite 207
LRB 6th floor mailroom
We are also available by appointment if you need to visit with us.
You can find here the newest Sample Ticket (Aug 2015) or a fillable pdf version to use for all DeepSeq library submissions except for the HiSeq4000 – that ticket is here, or use the HS4000 fillable pdf. To request a multiplexed run, please fill out the supplementary index form found here and submit it with your ticket. Please note that a sample submitted for sequencing is entered in the workflow and queue, which engages time and services. If you want the QC analysis only for your own information and are not certain you wish to move forward, please use the MBCL Fragment Analyzer or other QC services (e.g. Topo Cloning, Gel Purification).
For those preparing their own libraries, samples should be in ultrapure water or EB. We reserve the choice to hold back a sample which fails QC and contact you to discuss options. The use of a carrier agent during the final stages of workup is discouraged, as these can interfere with cluster formation and reduce the number of reads generated during sequencing.
For Illumina Library Building Notes, click here.
Library construction and data:
NOTE: It is VERY IMPORTANT that we receive the correct information about the primers and adapters used to build your library! Not all library types will run on all instruments or can be read in both directions. If you are using the Illumina-style indexes in the TruSeq/Nextera adapters, you must add a multiplex read step to detect your indexes. There is more information in the FAQ and Resources sections. On a related note, if you are doing a custom design to include adapters, your own index scheme, or custom sequencing primers, you must submit the results of your Topo cloning validation before we can run your samples. These sequences must span the P5 and P7 attachment sequences on either end of the library construct. If you do not agree to submit the sequence for verification, then you will be run with only the primers you request and at your own risk.
IF THERE IS A PROBLEM WITH YOUR RUN OR QUESTIONS ABOUT YOUR DATA, please let us know as soon as possible. The run metrics and machine files cannot be held for long because they are extremely large. The sooner we know there is a problem, the more likely we'll be able to help easily. Also, please note that in order to help sort out a problem we may ask a lot of questions and details about your sample and your analysis methods. This information is required in order for us to engage the tech support systems provided by our vendors. Please note that if the control lane on the run failed in any way, the entire flow cell is rerun. If your data is delivered to you, then the controls all passed spec., which means that the instrument functioned properly and the reagents and chemistry worked. That leaves us with investigating other issues including sample design, base balance, sizing, indexing, degraded DNA, as well as computational and analysis issues with the pipeline, etc. We'll do whatever we can to get things working, but the more information we have, the better. We are working on a trouble-shooting guide for libraries; it is under construction and is starting to come together here.
If you would like to consult with an applications specialist, please email us to get on the schedule.
Processing and analyzing a sample requires time and reagents. Payment for these services is the responsibility of the user submitting the sample and should be rendered in a timely fashion. In the event of a reagent or equipment failure, the samples will be rerun at the next possible opportunity at no additional charge.
Clients withdrawing samples prior to the analysis run will be charged a fee to recover QC assay costs. For the return of archived post-analysis samples, clients will be charged a delivery fee per sample.
~~~Inclement Weather Policy~~~
In case of extreme and/or inconvenient weather, the machines will still
be running but we may not be here for sample login or pickup. Please
email us to arrange drop-off if the weather is questionable.
The DSCL is closed when UMassMed is on "inclement weather alert“.