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Methodology

Ganesan, R, Leszyk, J., and Jacobson, A. Selective profiling of ribosomes associated with yeast Upf proteins. Methods 155: 58-67, 2019.

He, F., Celik, A., Baker, R., and Jacobson, A. High resolution profiling of NMD targets in yeast. In: Methods in Enzymology, Vol. 612: High-Density Sequencing Applications in Microbial Molecular Genetics (Editor: A.J. Carpoussis), Elsevier, San Diego, pp. 147-181, 2018. 

He, F., Amrani, N., Johansson, M.J.O., and Jacobson, A. Qualitative and quantitative assessment of the activity of the yeast NMD pathway. In: Methods in Enzymology Vol. 449. RNA Turnover (Editors: L. Maquat and M. Kiledjian), Elsevier, San Diego, pp.127-147, 2008. 

Wu, C., Amrani, N., Jacobson, A., and Sachs, M.S. The use of fungal in vitro systems for studying translational regulation. In: Methods in Enzymology Vol. 429. Translation Initiation: Extract Systems and Molecular Genetics (Editor: J. Lorsch), Elsevier, San Diego, pp. 203-225, 2007.

Ross, P.L., Huang, Y.N., Marchese, J.N., Williamson, B., Parker, K., Hattan, S., Khainovski, N., Pillai, S., Daniels, S.,  Purkayastha, S.,  Martin, S., Bartlet-Jones, M.,   He, F.,  Jacobson, A.,  and Pappin, D.J. Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents Mol. Cell. Proteomics 3: 1154-1169, 2004. 

Sachs, M.S., Wang, Z., Gaba, A., Fang, P., Belk, J., Ganesan, R., Amrani, N. and Jacobson, A. Toeprint analysis of the positioning of translation apparatus components at initiation and termination codons of fungal mRNAs. Methods 25: 105-114, 2002. 

Mangus, D.A. and Jacobson, A. Linking mRNA turnover and translation: assessing the polyribosomal association of mRNA decay factors and degradative intermediates. Methods 17: 28-37, 1999.

Parker, R., Herrick, D., Peltz, S.W., and Jacobson, A. Measurement of mRNA decay rates in Saccharomyces cerevisiae. In Methods in Enzymology: Molecular Biology of Saccharomyces cerevisiae (Eds. C. Guthrie, G. Fink), Academic Press, pp. 415-423, 1991.

Jacobson, A.  Purification and fractionation of poly(A)+ RNA.  In Methods in Enzymology, Volume 152:  Guide to Molecular Cloning Techniques (Eds: Berger, S.L. and Kimmel, A.R.), Academic Press, pp. 254-261, 1987. 

Steel, L.F., Ward, T.E., and Jacobson, A.  Intron Bypass:  A rapid procedure for eliminating introns from cloned genomic DNA and its application to a cellulase gene, Nucleic Acids Research 12: 5879-5895, 1984.

Palatnik, C.M., R.V. Storti and A. Jacobson.  Partial purification of a developmentally regulated mRNA from Dictyostelium discoideum by thermal elution from poly(U)-Sepharose.  J. Mol. Biol. 150, 389-398, 1981.