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Metabolism Core

Hyperglycemic clamp to assess insulin secretion and β-cell function in conscious mice

                                              Mouse Clamp

Following overnight fast, a 2-hr hyperglycemic clamp will be conducted in awake mice with a variable infusion of 20% glucose to raise and maintain plasma glucose concentration at ~300 mg/dl. Blood samples will be collected at 10~15 min intervals for the measurement of plasma glucose and insulin concentrations. The area-under-curve of plasma insulin levels and glucose infusion rate to maintain hyperglycemia will reflect pancreatic β-cell function in vivo.

Overview Mice2