National Mouse Metabolic Phenotyping Center 

The National Mouse Metabolic Phenotyping Center (MMPC) at UMass is designed to provide an array of sophisticated research techniques to the scientific community for the purpose of investigating mouse models of human disease. The mission of the UMass MMPC, funded by the National Institutes of Health (UC2-DK093000), is to advance medical and biological research by providing the scientific community with comprehensive, standardized, and high quality metabolic phenotyping services for mouse models of diabetes, diabetic complications, obesity and related disorders. The UMass MMPC is composed of multidisciplinary group of investigators and leading scientists with state-of-the-art technologies at the UMass Medical School and consists of the following Phenotyping Cores:

  1. Metabolism Core  performs elegant, physiologic, and non-invasive metabolic experiments to assess insulin sensitivity (GTT/ITT, hyperinsulinemic-euglycemic clamp), glucose/lipid/protein metabolism (using labeled isotopes), body composition (via 1H-MRS), energy balance (metabolic cages), and treadmill exercise in awake mice as well as drug trial studies with academic and pharmaceutical institutions.

  2. Analytical Core  utilizes Luminex, Cobas Clinical Chemistry Analyzer, and molecular experiments to perform a high-throughput, multiplexed analysis of serum/tissue levels of hormones, cytokines, chemokines and metabolites, liver function, and metabolic and inflammatory signaling pathways.

  3. Islet Core conducts sophisticated in vivo hyperglycemic clamp, ex vivo perifusion, and in vitro molecular studies to assess insulin secretion and pancreatic function as well as histological and morphological analysis of islet structure and mass.

  4. Cardiovascular Core applies state-of-the-art high-frequency, high-resolution digital imaging platform with color Doppler mode (VisualSonics Vevo2100) to perform 2-D and M-mode echocardiography to non-invasively assess cardiac structure/function, ECG/blood pressure, and vascular/endothelial function as well as elegant micro-surgery to generate CVD and PVD models to investigate diabetic complications.

  5. The Humanized Mouse Cell Transplantation and Assessment Core offers highly unique humanized mice to the scientific community, and expert and standardized techniques to assess in vivo function of transplanted human islets and stem cell-derived b-cells in immunodeficient mice.