Search Close Search
Search Close Search
Page Menu

Metabolic Services

The UMass Metabolic Disease Research Center (MDRC) conducts specialized and standardized metabolic experiments to measure insulin sensitivity, glucose/lipid metabolism, pancreatic β-cell function, glucagon action, body composition, energy balance, and exercise capacity. Most experiments are performed in physiological states and in awake mice. The metabolic services include:

  • Hyperinsulinemic-euglycemic clamp to assess organ-specific insulin sensitivity and glucose metabolism in awake mice
  • Hyperglycemic clamp to assess pancreatic b-cell function and insulin secretion in awake mice
  • Glucose tolerance test (GTT), GTT with insulin secretion, and insulin tolerance test (ITT)
  • In vivo measurement of glucose and lipid metabolism in individual organs using labeled metabolites in awake mice
  • Glucagon clamp to assess organ-specific glucagon action in awake mice
  • Noninvasive measurement of whole body and tissue composition of fat/lean/water mass using 1H-MRS
  • Noninvasive analysis of food/water intake, energy expenditure, respiratory exchange ratio, and physical activity at controlled temperature (cold acclimation, thermoneutrality) and light:dark cycle (circadian rhythm) using metabolic cages with an environmental chamber (TSE Systems)
  • Survival surgery of jugular vein cannulation for in vivo experiments

     ·     Chronic and acute induction of obesity, insulin resistance, and type 1 and type 2 diabetes using specialized diets (e.g., high-fat diet, high-fructose diet), lipid/fructose infusion, streptozotocin injection, and phloridzin treatment



Overview Mice2