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Useful Links

Educational and Technical Links:

Education

International Society for Advancement of Cytometry - ISAC
http://www.isac-net.org

New England Cytometry
http://newenglandcytometry.com

Purdue University Flow and Image Cytometry
http://www.cyto.purdue.edu

http://www.cyto.purdue.edu/class/

http://www.cyto.purdue.edu/flowcyt/educate.htm

http://www.cyto.purdue.edu/flowcyt/labinfo/labinfo.htm

Maeckerlab Weblog - Research and training materials for flow cytometry and immune monitoring.
http://maeckerlab.typepad.com

BD Biosciences Educational Programs Online

http://www.bdbiosciences.com/us/s/training/e-learning/e-learningcourses for beginners

http://static.bdbiosciences.com/training/diva6_course/index.html?_ga=2.221200155.225408873.1527001100-1298382617.1506082957 for Diva training

http://www.bdbiosciences.com/us/s/webinars/archives

Howard M. Shapiro, MD.: Practical Flow Cytometry, 4th Edition (free ebook)
https://www.beckman.com/resources/reading-material/ebooks/practical-flow-cytometry

Tools

BD Product Selection Tools

https://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools

BD Biosciences Spectral Viewer
https://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer

Cytek Spectrum Viewer
https://cytekbio.com/blogs/resources/tagged/tools

Thermo Fisher Spectra Viewer
https://www.thermofisher.com/us/en/home/life-science/cell-analysis/labeling-chemistry/fluorescence-spectraviewer.html

FluoroFinder
Design Experiments for UMASS Flow Core's cytometers’ laser and filter configurations.
https://umassmed.fluorofinder.com

Live/Dead Staining
Dead cells bind antibody non-specifically and can exhibit a higher autofluorescense. Thus it is very important to exclude dead cells from your analysis - or sorted cells to improve the quality of your experiment. There are a wide array of options to choose from. They range from the classic dyes (PI, DAPI, Hoechst or 7AAD) to the newer fixable live/dead stain versions.

Below you will find a selection of companies providing these dyes as well as offering very good explanations on how those dyes work and give examples.

Thermo Fisher LIVE/DEAD Fixable Dead Cell Stains
LIVE/DEAD Fixable Blue stain - UV
LIVE/DEAD Fixable Violet stain - 405
LIVE/DEAD Fixable Aqua stain - 405
LIVE/DEAD Fixable Yellow stain - 405
LIVE/DEAD Fixable Green stain - 488
LIVE/DEAD Fixable Red stain - 488 or 561
LIVE/DEAD Fixable Far Red stain - 633
LIVE/DEAD Fixable Near-IR stain - 633

Biotium Live-or-Dye™ Fixable Viability Stains
Live-or-Dye Fixable Viability Stains
Live-or-Dye NucFix™ Red, a unique cell membrane impermeable dye that specifically stains the nuclei of dead cells, labeled covalently and does not transfer between cells after fixation.

Bio-Rad Live-Dead Exclusion Stains
VivaFix Fixable Viability Dyes
ReadiDrop 7-AAD
ReadiDrop PI
PureBlu DAPI
PureBlu Hoechst 33342

BioLegend Live-Dead Zombie Stains
Zombie Fixable Dyes
Helix NP NIR (ex/em - 640nm/660nm)
Draq7 (ex/em - 633nm/695nm)
PI (ex/em - 493nm/636nm)
7AAD (ex/em - 546nm/647nm)

Abcam live-and-dead-cell-assay
Live and Dead Cell Assay

Filtration recommendations
- Sterile 12x75 mm tubes with filter caps; by Corning - Fisher Cat. number 08-771-23
- Nitex Nylon Filtration Mesh order from: ELKO Filtering Co, LLC. (elkofiltering.com) Nylon mesh roll 37µ opening size, 40" wide, sold by the yard) Item#: 03-37/24
- Miltenyi MACS^® SmartStrainers #130-098-458