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Useful Links

Educational and Technical Links:


International Society for Advancement of Cytometry - ISAC

New England Cytometry

Purdue University Flow and Image Cytometry

Maeckerlab Weblog - Research and training materials for flow cytometry and immune monitoring.

BD Biosciences Educational Programs Online for beginners for Diva training

Howard M. Shapiro, MD.:  Practical Flow Cytometry, 4th Edition (free ebook)


BD Product Selection Tools

BD Biosciences Spectral Viewer

Cytek Spectrum Viewer

Thermo Fisher Spectra Viewer

Design Experiments for UMASS Flow Core's cytometers’ laser and filter configurations.

Live/Dead Staining
Dead cells bind antibody non-specifically and can exhibit a higher autofluorescense. Thus it is very important to exclude dead cells from your analysis - or sorted cells to improve the quality of your experiment. There are a wide array of options to choose from. They range from the classic dyes (PI, DAPI, Hoechst or 7AAD) to the newer fixable live/dead stain versions.

Below you will find a selection of companies providing these dyes as well as offering very good explanations on how those dyes work and give examples.

Thermo Fisher LIVE/DEAD Fixable Dead Cell Stains
LIVE/DEAD Fixable Blue stain - UV
LIVE/DEAD Fixable Violet stain - 405
LIVE/DEAD Fixable Aqua stain - 405
LIVE/DEAD Fixable Yellow stain - 405
LIVE/DEAD Fixable Green stain - 488
LIVE/DEAD Fixable Red stain - 488 or 561
LIVE/DEAD Fixable Far Red stain - 633
LIVE/DEAD Fixable Near-IR stain - 633

Biotium Live-or-Dye™ Fixable Viability Stains
Live-or-Dye Fixable Viability Stains
Live-or-Dye NucFix™ Red, a unique cell membrane impermeable dye that specifically stains the nuclei of dead cells, labeled covalently and does not transfer between cells after fixation.

Bio-Rad Live-Dead Exclusion Stains
VivaFix Fixable Viability Dyes
ReadiDrop 7-AAD
ReadiDrop PI
PureBlu DAPI
PureBlu Hoechst 33342

BioLegend Live-Dead Zombie Stains
Zombie Fixable Dyes
Helix NP NIR (ex/em - 640nm/660nm)
Draq7 (ex/em - 633nm/695nm)
PI (ex/em - 493nm/636nm)
7AAD (ex/em - 546nm/647nm)

Abcam live-and-dead-cell-assay
Live and Dead Cell Assay

Filtration recommendations
- Sterile 12x75 mm tubes with filter caps; by Corning - Fisher Cat. number 08-771-23
- Nitex Nylon Filtration Mesh order from: ELKO Filtering Co, LLC. ( Nylon mesh roll 37µ opening size, 40" wide, sold by the yard) Item#: 03-37/24 
- Miltenyi MACS® SmartStrainers  #130-098-458

Corporate – Instruments and reagents…


Amnis ImageStream Cytometer


BD Biosciences    





Diatec Antibodies

eBioscience Antibodies

Evergreen Laser Corporation

Linscott's Antibody Directory


Miltenyi Biotec

Novus Biologicals

Selleck Chemicals

Sony Biotechnology


VWR Antibody Selector

Treestar (FlowJo) software