DeepSeq Core Service Survey - UMass Chan Medical School

Deep Sequencing Goals

This section's aim is to discover your goals when using Next-Gen Sequencing.

Have you used Deep-Seq / Next-Gen Sequencing before from any provider? * Yes No

What is the biggest barrier preventing you from using Deep Sequencing? * Cost Time or skill set for library prep Insufficient starting material Data analysis

Which of the following support options would you find helpful in choosing Deep Sequencing services? Please check all that apply. * Consult with Core director about experimental design and technical issues Consult with Core director focused on cost-benefit (such as at what point to choose DeepSeq over Taqman or microarray) Consult with an Illumina specialist A regularly available on-campus Illumina specialist Consult with a third-party library prep kit specialist (i.e. NEB or Takara) Lab visit for benchtop help from an Illumina Field Application Specialist

If you have done Deep Sequencing in the past, from which provider(s) have you gotten service? Please choose all that apply. * N/A - have not used Department or lab personal instrument UMassMed Core Facility Outside provider (such as BGI or Novogene)

Why do or would you choose a particular option when selecting Deep Sequencing services? Please choose your top priority. * Speed Read length Read depth Price Ease of analysis Other/comment

Please tell us about your priorities when choosing DeepSeq Services:

Which of the following is more important to your goals when choosing a run type? * Number of total reads Number of unique reads Neither

Performing an extra bead cleanup on a Deep Sequencing library can increase the number of reads you get back. Does that knowledge interest you in any of the following options? * Performing an extra cleanup during my prep Paying for an extra cleanup as a Core service None of the above

Where do you currently get your data analysis services? Please check all that apply. * N/A - do not use Deep Sequencing Myself or in my lab using our own pipeline Myself or in my lab using commercial software UMassMed Bioinformatics Core Outside provider that does my sequencing Outside provider for bioinformatics only

Are you satisfied with the data analysis services that you currently use? * N/A - do not use Deep Sequencing Yes No

Please tell us what your data analysis needs are and which data analysis services have been difficult to find or use:

This section asks about Deep Sequencing methods and topics that you are interested in

Which of the following DeepSeq methods are you interested in / already using? * ATAC-Seq Guide-Seq Cut & Run Single-cell such as Chromium 10X Low-input libraries MetSeq Other None of the above

What other method(s) are you using or interested in learning more about?

Would you want to attend a seminar, hear more information, or want to receive notifications of special products related to your method of interest? Seminar Email more info Product Notifications

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~Welcome to the Core Labs survey~

Deep Sequencing Goals

This section asks about Deep Sequencing methods and topics that you are interested in