IXM High Throughput Imaging Core
The UMass Chan HTI core facility utilizes state of the art technology to acquire large amounts of high quality imaging data for use by the UMass Chan community. The HTI core employs the ImageXpress Micro-XL to image experiments in plates or on slides by immunofluorescence and/or transmitted light with various objectives (2x to 100x). In conjunction with the IXM, the Catalyst CRS-5 robot enables researchers high throughput capabilities by enabling the acquisition of 45 plates in a single session. For custom automated analysis of the data, MetaMorph software allows custom analysis journals, macros and many other user specified options. The HTI core has three separate work stations for analysis of data which can be accessed locally or remotely enabling researchers to access their data from anywhere. This platform can be used for structural analysis of cells, multi-wavelength cell scoring when assaying cells for various markers, analysis of populations of worms along with many other user driven applications
HIGH THROUGHPUT IMAGING CORE IS NOW CLOSED. SMALL MOLECULE SCREENING HAS TAKEN OVER SERVICES. FOR MORE INFORMATION PLEASE CONTACT SANGRAM PARELKAR - 508-856-8315
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Instrumentation capabilities
IXM-XL
Compatible with: slides, 96, 384, and 1536-well plates
Objectives: 2X, 4X, 10X, 20X, 40X (0.95NA), 100X air (0.95NA)
Filter sets: DAPI, FITC, CY5, CFP, YFP, Texas Red
Transmitted light option
Catalyst CRS-5 robot
Automated handling of up to 45 plates to enable high throughput data acquisition
Pricing and availability
$345 for training for up to 6 persons
$35/hr IXM-XL
$12/hr for use of the analysis stations
$4,025/year full access for up to 6 users per la
Available to all UMass Chan researchers
Contact
Abraham Brass, Abraham.Brass@umassmed.edu (Primary Contact)
George Savidis, George.Savidis@umassmed.edu
Jennifer Benanti, Jennifer.Benanti@umassmed.edu