Molecular Devices SpectraMax Plus 384 Microplate Reader (B)

  Molecular Devices SpectraMax Plus 384 Microplate Reader (B)   location: AS8-1028   status: in service   reserve use
UV-visible absorbance reader for plates with up to 384-well plates and individual cuvettes. Tunable monochromator allows for reading as many as 6 wavelengths/sample in the range of 190nm - 1000nm in both end-point and kinetic assays.

Applications

Essentially, all UV/VIS spectrophotometric assays that are read in a conventional spectrophotometer or microplate reader can be read in the SpectraMax Plus384:

• DNA/RNA/protein quantitation and purity
• Colorimetric assays
• ELISAs/EIAs
• Immunoassays
• Drug dissolution profiles
• Enzyme kinetics (e.g., Ki, Km, etc.)
• Kinetic turbidimetric methods
• ADME-Tox assays
• Lipophilicity assays
• Human Serum Albumin binding assays
• Permeability assays (PAMPA)
• Solubility assays

Performance Specifications

Wavelength Range: 190 - 1000 nm
Wavelength Selection: Monochromator, tunable 1.0 nm increments
Wavelength Bandwidth: 2 nm
Wavelength Accuracy: < ± 1.0 nm
Wavelength Repeatability: ± 0.2 nm
Photometric Range: 0.0 to 4.0 OD
Photometric Resolution: 0.001 OD
Photometric Accuracy (Microplate): < ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Accuracy (Cuvette): < ± 0.005 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Precision: < ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Baseline Flatness: < 0.001 OD
Stray Light: < 0.05% @ 230 nm
Light Source: Xenon flash lamp
Microplate Read Time:
Normal Mode
96 wells. . . . 9 seconds
384 wells. . . . 29 seconds
Speed Read Mode
96 wells. . . . 5 seconds
384 wells. . . .16 seconds
Temperature Range: Ambient + 4 °C up to 45 °C
Temperature Uniformity (Microplate): ± 0.5 °C @ 37 °C 

Download documentation: SoftMax Pro 5 User Guide.pdf    SoftMax Pro Forumla Guide.pdf