Molecular Device SpectraMax Plus 384 Microplate Reader (A)
Molecular Devices Spectra Max Plus 384 Microplate Reader (A) location: AS8-2009A status: in service reserve use
UV-visible absorbance reader for plates with up to 384-well plates and individual cuvettes. Tunable monochromator allows for reading as many as 6 wavelengths/sample in the range of 190nm - 1000nm in both end-point and kinetic assays.
Applications
Essentially, all UV/VIS spectrophotometric assays that are read in a conventional spectrophotometer or microplate reader can be read in the SpectraMax Plus384:
- DNA/RNA/protein quantitation and purity
- Colorimetric assays
- ELISAs/EIAs
- Immunoassays
- Drug dissolution profiles
- Enzyme kinetics (e.g., Ki, Km, etc.)
- Kinetic turbidimetric methods
- ADME-Tox assays
- Lipophilicity assays
- Human Serum Albumin binding assays
- Permeability assays (PAMPA)
- Solubility assays
Performance Specifications
Wavelength Range: 190 - 1000 nm
Wavelength Selection: Monochromator, tunable 1.0 nm increments
Wavelength Bandwidth: 2 nm
Wavelength Accuracy: < ± 1.0 nm
Wavelength Repeatability: ± 0.2 nm
Photometric Range: 0.0 to 4.0 OD
Photometric Resolution: 0.001 OD
Photometric Accuracy (Microplate): < ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Accuracy (Cuvette): < ± 0.005 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Precision: < ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Baseline Flatness: < 0.001 OD
Stray Light: < 0.05% @ 230 nm
Light Source: Xenon flash lamp
Microplate Read Time:
Normal Mode
96 wells. . . . 9 seconds
384 wells. . . . 29 seconds
Speed Read Mode
96 wells. . . . 5 seconds
384 wells. . . .16 seconds
Temperature Range: Ambient + 4 °C up to 45 °C
Temperature Uniformity (Microplate): ± 0.5 °C @ 37 °C
Documentation downloads: SoftMax Pro 5 User Guide.pdf SoftMax Pro Forumla Guide.pdf