Research
Discovery of Cas9 Orthologs
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AAV Delivery
Ibraheim et al. 2018 Genome Biology
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Anti-CRISPR
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Modified crRNAs
Mir et al. 2018 Nature Communications
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Site-Specific Proteomics
Gao et al. 2018 Nature Methods
Genome organization and subnuclear protein localization are essential for normal cellular function and have been implicated in gene expression, DNA replication and genomic stability. Recently, we combined the RNA-programmable, nuclease-dead CRISPR-Cas9 (dCas9) platform with enzymatic labeling for site-specific identification of protein spatially tied to a repetitive locus of interest. This novel chromatin mapping tool, dCas9–APEX2 biotinylation at genomic elements by restricted spatial tagging (C-BERST), successfully characterized known centromeric and telomeric protein binding and revealed putative factors associated with these genomic regions.