We study the regulation and the activity of enzymes that remodel chromatin and how these enzymes promote normal tissue differentiation.
Discovering how cell differentiation is regulated is critical to understanding fundamental biology and to proposing new treatment methods for differentiation-centered diseases, including cancer.
We utilize molecular biology, biochemistry, and epigenetic experimental techniques to study mammalian SWI/SNF-mediated chromatin remodeling.
We are a friendly and collaborative team of researchers.
Muscle-specific pyruvate kinase isoforms, PKM1 and PKM2, regulate mammalian SWI/SNF proteins and histone 3 phosphorylation during myoblast differentiation
Olea-Flores M, Sharma T, Verdejo-Torres O, DiBartolomeo I, Thompson PR, Padilla-Benavides T, Imbalzano AN.
FASEB J. 2024 Jun 15;38(11):e23702. doi: 10.1096/fj.202400784R. PMID: 38837439; PMCID: PMC11268309.
The Bromodomains of the mammalian SWI/SNF (mSWI/SNF) ATPases Brahma (BRM) and Brahma Related Gene 1 (BRG1) promote chromatin interaction and are critical for skeletal muscle differentiation.
Sharma T, Robinson DCL, Witwicka H, Dilworth FJ, Imbalzano AN.
Nucleic Acids Res. 2021 Aug 20;49(14):8060-8077. doi: 10.1093/nar/gkab617. PMID: 34289068; PMCID: PMC8373147.
Calcineurin Broadly Regulates the Initiation of Skeletal Muscle-Specific Gene Expression by Binding Target Promoters and Facilitating the Interaction of the SWI/SNF Chromatin Remodeling Enzyme
Witwicka H, Nogami J, Syed SA, Maehara K, Padilla-Benavides T, Ohkawa Y, Imbalzano AN.
Mol Cell Biol. 2019 Sep 11;39(19):e00063-19. doi: 10.1128/MCB.00063-19. PMID: 31308130; PMCID: PMC6751634.
Opposing calcium-dependent signalling pathways control skeletal muscle differentiation by regulating a chromatin remodelling enzyme.
Nasipak BT, Padilla-Benavides T, Green KM, Leszyk JD, Mao W, Konda S, Sif S, Shaffer SA, Ohkawa Y, Imbalzano AN.
Nat Commun. 2015 Jun 17;6:7441. doi: 10.1038/ncomms8441. PMID: 26081415; PMCID: PMC4530624.
