Our research is focused on improving preservation of DCD (donors after cardiac death) liver grafts with machine perfusion. In addition, we are testing for the first time if gene silencing using si-RNAs during machine perfusion preservation could reduce ischemia reperfusion injury. Machine perfusion preservation allows graft viability and may improve transplant logistics by extending the period for organ allocation and transport. It may also be able to “resuscitate” grafts that are initially deemed not transplantable. Through this innovative translational approach our lab is performing experiments that could have a major clinical impact for a widespread and relevant problem. There is potential to increase the pool of livers for transplant, reduce the mortality on the waiting list and reduce morbidity and mortality of thousands patients after liver transplant.