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Publications

  1. Yu, C., Wang, C., Forman, T., Xie, J., Major, S., Fang, M.X., Voyer, J.E., Pogliano, J., Fu, T.M. 2025, Phage SSB detection by retron Eco8 msDNA unleashes nuclease-mediated immunity. Molecular Cell, in press
  2. Wang, C., Rish, A. D., Armbruster, E. G., Xie, J., Loveland, A. B., Shen, Z., Gu, B., Korostelev, A.A., Pogliano, J., & Fu, T. M. 2025, Disassembly activates Retron-Septu for antiphage defense. Science, 389: eadv3344.
  3. Fosuah, E., Shen, Z., Xie, J., Wang, C., Lin, Q., & Fu, T. M. 2025, Assembly and activation of the death-inducing signaling complex. Proc. Natl. Acad. Sci. USA, 122(23), e2504819122.
  4. Zheng, W., Lowry, A. J., Smith, H. E., Xie, J., Rawson, S., Wang, C., Ou, J., Sotomayor, M., Fu, T.M., Yang, H., & Holt, J. R. 2025, Structural and functional basis of mechanosensitive TMEM63 channelopathies. Neuron., 113 (15): 2474-89.
  5. Rish, A.D., Fosuah, E., Shen, Z., Marathe, IA., Wysocki, VH., Fu, T.M. 2025, Architecture remodeling activates the HerA-DUF anti-phage defense system. Molecular Cell, 85 (6): 1189-1201.
  6. Yang, X.Y., Shen, Z., Wang, C., Nakanishi, K., Fu, T.M. 2024, DdmDE eliminates plasmid invasion by DNA-guided DNA targeting. Cell, 187 (19): 5253-66.
  7. Yang, X.Y., Shen, Z., Xie, J., Greenwald, J., Marathe, I., Lin, Q., Xie, W.J., Wysocki, V.H., Fu, T.M. 2024, Molecular basis of Gabija anti-phage supramolecular assemblies. Nat. Struct. Mol. Biol., 31:1243-50.
  8. Wang, C., Shen, Z., Yang, X.Y., Fu, T.M. 2024, Structures and functions of short Argonautes. RNA biology, 21: 1-7.
  9. Li, Y., Shen, Z., Zhang, M., Yang, X.Y., Cleary, S.P., Xie, J., Marathe, I.A., Greenwald, J., Rish, A.D., Wysocki, V.H., Chen, C., Chen, Q.#, Fu, T.M.#, Yu, Y.# 2024. PtuA and PtuB assemble into an inflammasome-like oligomer for anti-phage defense. Nat. Struct. Mol. Biol., 31:413-423. (#Co-corresponding author).
  10. Cruz, A., Nakano, H., Chou, S., Krantz, M., Fu, T.M., Felicelli, C., Wu. H., Ombrello, M. 2024, Novel loss of function mutations in the death domain of tumor necrosis factor superfamily receptor 1A (TNFRSF1A) in children with systemic juvenile idiopathic arthritis (sJIA). Immunol., 212: 0994_5470
  11. Shen, Z., Lin, Q., Yang, X.Y., Fosuah, E., Fu, T.M. 2023, Assembly-mediated activation of the SIR2-HerA supramolecular complex for anti-phage defense. Molecular Cell, 83: 4586-99.
  12. Shen, Z., Yang, X.Y., Xia, S., Huang, W., Taylor, D., Nakanishi, K., Fu, T.M. 2023. Oligomerization-mediated activation of a short prokaryotic Argonaute. Nature, 621: 154-61.
  13. Zheng, W., Rawson, S., Shen, Z., Smith, H., Halford, J., Shen, C., Murthy, S., Ulbrich, M., Sotomayor, M., Fu, T.M.#, Holt, J.R.# TMEM63 proteins function as monomeric mechanosensitive ion channels. Neuron, 111:3195-3210. (#Co-corresponding author).
  14. Rish, A.D., Shen, Z., Chen, Z., Zhang, N., Zheng, Q., Fu, T.M. Molecular mechanisms of Holliday junction branch migration catalyzed by an asymmetric RuvB hexamer. Nat. Commun., 14:3549.
  15. Cheng, J., Yu, Y., Wang, X., Zheng, X., Liu, T., Hu, D., Jin, Y., Lai, Y., Fu, T.M., Chen, Q. 2023, Structural basis for the self-recognition of sDSCAM in Chelicerata. Nat. Commun., 14:2522.
  16. Rish, A.D., Fu, T.M. 2023. CRISPR-Cas has a new juggling act: interplay between nuclease and protease. Nat. Struct. Mol. Biol., 30:126-128.
  17. Solebo, O., Lin, L., Nwankwo, I., Zhou, J., Fu, T.M., Ke, H. 2023. Plasmodium falciparum utilizes pyrophosphate to fuel an essential proton pump in the ring stage and the transition to trophozoite stage. PLOS. Pathogens., 19: e1011818.
  18. Udinn, N., Binzel, D., Shu, D., Fu, T.M., Guo,P. 2023.Targeted delivery of RNAi to cancer cells using RNA-ligand displaying exosome. Pharm. Sin. B, 83(1): 74-89.
  19. Jiang, C., Dai, X., He, S., Zhou, H., Fang, L., Guo, J., Liu, S., Zhang, T., Pan, W., Yu, H., Fu, T.M., Li, D., Inuzuka, H., Wang, P., Xiao, J., Wei, W. 2022. Ring domains are essential for GATOR2-dependent mTORC1 activation. Molecular Cell, 83(1): 74-89.
  20. Wang, L., Wu, D., Robinson, C., Fu, T.M. Identification of mEAK-7 as a human V-ATPase regulator via cryo-EM data mining. Proc. Natl. Acad. Sci. USA, 119: e2203742119.
  21. Fontana, P., Dong, Y., Pi, X., Tong, A.B., Hecksei, C.W., Wang, L., Fu, T.M., Bustamante, C., Wu, H. 2022. Structure of cytoplasmic ring of nuclear pore complex by integrative cryo-EM and AlphaFold. Science, 376: abm9326
  22. Zheng, W., Shen, C., Wang, L., Rawson, S., Xie, W.J., Nist-Lund, C., Wu, J., Shen, Z., Xia, S., Holt, J.R., Wu, H., Fu, T.M. 2022. pH regulates potassium conductance and drives a constitutive proton current in human TMEM175. Science Adv., 8 (12): abm1568
  23. Rish, A.D., Shen, Z., Fu, T.M. It takes two to tango: two gates orchestrate the opening of human TRPM2. Cell Calcium, 101: 102523
  24. Zhao, J.S., Shi, S., Qu, H.Y., Keckesova, Z., Cao, Z.J., Yang, L.X., Yu, X., Feng, L., Shi, Z., Krakowiak, J., Mao, R.Y., Shen, Y.T., Fan, Y.M., Fu, T.M., Ye, C., Xu, D., Gao, X., You, J., Li, W., Liang, T., Lu, Z., Feng, Y.X. 2022. Glutamine synthetase licenses APC/C-mediated mitotic progression to drive tumor growth. Metab., 4: 239-253
  25. Vora, S.M., Fontana, P., Leger, V., Zhang, Y., Fu, T.M., Lieberman, J., Gehrke, L., Shi, M., Wang, L., Wu, H. 2022. Targeting stem-loop 1 of the SARS-CoV-2 5’UTR to suppress viral translation and Nsp1 evasion. Proc. Natl. Acad. Sci., 119 (9): e2117198119
  26. Shen, C., Li, R., Negro, R., Cheng, J., Vora, S., Fu, T.M., Wang, A., He, K., Andreeva, L., Gao, P., Tian, Z., Favell, R.A., Zhu, S., Wu, H. 2021. Phase separation drives RNA virus-induced activation of the NLRP6 inflammasome. Cell, 184: 5759-74.
  27. Xia, S., Zhang, Z., Magupalli, V., Pablo, J.L., Dong, Y., Vora, S., Wang, L., Fu, M., Jacobson, M.P., Greka, A., Lieberman, J., Ruan, J., Wu, H. 2021. Gasdermin D pore structure reveals preferential release of mature interleukine-1. Nature, 593: 607-611.
  28. Xia, S., Chen, Z., Shen, C., Fu, T.M. Higher-order assemblies in immune signaling: supramolecular complexes and phase separation. Protein Cell, 12 (9): 680-694.
  29. Wu, C., Tang, D., Cheng, J., Hu, D., Yang, Z., Ma, X., He, H., Yao, S., Fu, T.M.#, Yu, Y.#, Chen, Q.#Mechanisms of spacer acquisition by sequential assembly of the adaptation module in Synechocystis. Nucleic Acids Res., 49 (5): 2973-84. (#Co-corresponding author)
  30. Wang, L., Chen, Z., Wu, H., Fu, T.M. 2021. Purification and cryo-electron microscopy structure determination of human V-ATPase. STAR Protoc., 2 (1):100350
  31. Hollingsworth, L.R., David, L., Li, Y., Griswold, A.R., Ruan, J., Sharif, H., Fontana, P., Orth-He, E.L., Fu, T.M., Bachovchin, D.A., Wu, H. Mechanism of filament formation in UPA-promoted CARD8 and NLRP1 inflammasomes. Nat. Commun., 12 (1): 1-13.
  32. Wang, L., Wu, D., Robinson, C., Wu, H.#, Fu, T.M.#, 2020, Structures of a complete human V-ATPase reveal mechanisms of its assembly. Molecular Cell, 80: 1-11 (#Co-corresponding author)
  33. Yin, L., Masuyer, G., Zhang, S., Zhang, J., Miyashita, S.I., Burgin, D., Lovelock, L., Coker, S.F., Fu,M., Stenmark, P., Dong, M. 2020. Characterization of a membrane binding loop leads to engineering botulinum neurotoxin B with improved therapeutics efficacy. PLoS. Biol., 3: e3000618
  34. Xia, S., Wang, L., Fu, T.M.#, Wu, H.#, 2019, Mechanism of TRPM2 channel gating revealed by cryo-EM. FEBS J., 288 (17):3333-39 (#Co-corresponding author)
  35. Pan, L.*, Fu, T.M.*, Zhao, W.*, Zhao, L.*, Chen, W., Qiu, C., Liu, W., Liu, Z., Piai, A., Fu, Q., Chen, S., Wu, H., Zhou, J. Higher-order clustering of the transmembrane anchor of DR5 drives signaling. Cell, 176 (6): 1477-89 (*Co-first author)
  36. Li, Y.R., Chen, M., Zhang, J., Lin, S.Y., Ishikawa, T., Monteleone, E., Papa, A., Chiang, S.Y., Chen, H., Fu, T.M., Hsu, C.H., Katon, J., Menon, A.V., Fung, J., Berry, K., Wan, L., Teruya-Feldstein, J., Wu, H., Shi, Y., Matesic, L., Chen, R., Wei, W., Pandolfi, P.P. Reactivation of the PTEN tumor suppressor for cancer prevention and treatment. Science, 364, eaau0159.
  37. Shen, C., Lu, A., Xie, W., Ruan, J., Negro, R., Fu, T.M. #, Wu, H. # Molecular mechanism of NLRP6 inflammasome assembly and activation. Proc. Natl. Acad. Sci. USA, 116 (6): 2052-57 (#Co-corresponding author)
  38. Wang, L.*, Fu, T.M.*#, Zhou, Y., Xia, S., Greka, A., Wu, H.# Structures and gating of human TRPM2. Science, 362, eaav4809 (*Co-first author; #Co-corresponding author)
  39. Li, Y.*, Fu, T.M.*#, Lu, A., Witt, K., Ruan, J., Shen, C., Wu, H.# Cryo-EM structures of ASC and NLRC4 CARD filaments reveal a unified mechanism of nucleation and activation of caspase-1. Proc. Natl. Acad. Sci. USA, 115 (43):10845-52 (*Co-first author; #Co-corresponding author)
  40. Luong, P., Hedl, M., Yan, J., Zuo, T., Fu, T.M., Jiang, X., Thiagarajah, J., Hansen, S.H., Lesser, C.F., Wu, H., Abraham, C., Lencer, W.T. INAVA-ARNO complexes bridge mucosal barrier function with inflammatory signaling. eLife, 7: e38539
  41. Xia, S., Fu, T.M., Wu, H. Inflammation NODs to Antagonists of RIP2-XIAP interaction. Mol. Cell, 69(4):535-36.
  42. Liu, P., Gan, W., Su, S., Hauenstein AV., Fu, T.M., Brasher, B., Schwerdtfefer C., Liang, A.C., Xu, M., Wei, W. K63-linked polyubiquitin chains bind to DNA to facilitate DNA damage repair. Sci. Signal., 11(533): 1-12
  43. Fu, T.M., Shen, C., Li, Q., Zhang, P., Wu, H. Mechanism of ubiquitin transfer promoted by the TRAF6 RING Dimer. Proc. Natl. Acad. Sci. USA, 115(8): 1783-88.
  44. Fu, T.M., Li, Y., Lu, A., Li, Z., Vajjhala, P.R., Cruz, A.C., Srivastava, D.B., DiMiao, F., Penczek, P.A., Siegel, R.M., Stacey, K.J., Egelman, E.H., Wu, H. Cryo-EM structure of Caspase-8 tandem DED filament reveals assembly and regulation mechanisms of the death-inducing signaling complex. Mol. Cell, 64: 236-50. (Feature Article of the Issue)
  45. Fu, Q.*, Fu, T.M.*, Cruze, A., Richardson, T., Wu, H., Zhou, J. 2016. Structural basis and functional role of intramembrane trimerization of the Fas/CD95 death receptor. Mol. Cell, 61: 602-613 (*co-first author)
  46. Lu, A., Li, Y., Schmidt, F., Yin, Q., Chen, S., Fu, T.M., Tong, A.B., Ploegh, HL., Mao, Y., Wu, H. Molecular basis of Caspase-1 polymerization and its inhibition by a new capping mechanism. Nat. Struct. Mol. Biol., 23: 416-423.
  47. Ru, H., Chambers, M.G., Fu, T.M., Tong, A.B., Liao, M., Wu, H. Molecular mechanism of V(D)J recombination from synaptic RAG1-RAG2 complex structures. Cell, 163: 1138-1152
  48. Yin, Q. *, Fu, T.M. *, Li, J., Wu, H. Structural biology of innate immunity. Annual. Rev. Immunol., 33:393-416. (*co-first author)
  49. Lu, A., Kabaleeswaran, V., Fu, T.M., Magupalli, VG, Wu, H. Crystal structure of the F27G AIM2 PYD mutant and similarities of its self-association to DED/DED interactions. J. Mol. Biol., 426: 1420-7
  50. Li, D. *, Fu, T.M. *, Nan, J., Liu, C., Li, L.F., Su, X.D. Structural basis for the autoinhibition of the C-terminal kinase domain of human RSK1. Acta Crystallogr. Sec. D Biol. Cryst., 68:680-5 (*co-first author)
  51. Fu, T.M., Almqvist, J., Liang, Y.H., Li, L., Huang, Y., Su, X.D. Crystal structures of cobalamin-independent methionine synthase (MetE) from Streptococcus mutans : a dynamic zinc-inversion model. J. Mol. Biol., 412: 688-697.
  52. Feng, MJ, Fu, T.M., Liu, X., Li, L. Purification, crystallization and preliminary X-ray analysis of SMU.1108c protein from Streptococcus mutans. Acta Crystallogr. Sec. F Struct. Biol. Cryst. Commun., 67:76-78.
  53. Fu, T.M., Liu, X., Li, L., Su, X.D. The structure of the hypothetical protein smu.1377c from Streptococcus mutans suggests a role in tRNA modification. Acta Crystallogr. Sec. F Struct. Biol. Cryst. Commun., 66:771-775.
  54. Fu, T.M., Li, D., Nan, J., Li, L., Xue, Y., Su, X.D. Protein preparation, crystallization and preliminary X-ray analysis of the C-terminal kinase domain of human Rsk1 serine/threonine protein kinase. Acta Crystallogr. Sec. F Struct. Biol. Cryst. Commun., 63:1026-1028.
  55. Fu, T.M., Zhang, X.Y., Li, L., Liang, Y.H., Su, X.D. Preparation, crystallization and preliminary X-ray analysis of methionine synthase (MetE) from Streptococcus mutans. Acta Crystallogr. Sec. F Struct. Biol. Cryst. Commun., 62:984-985.