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Proteomics and Intact Protein Analysis

Rates effective July 1, 2023
    Rates (per hour)  
 

General Rates
 UMass
External
Academic
Corporate
  
 
Staff Scientist
 $100
$150
$200
  
 
Director
 $150
$225
$300
  
 
Instrument Time
 $155
$210
$315
  
       
    Rates (per sample)  
 

Proteomics (LC-MS/MS)
UMass
External
Academic
Corporate
  
 
Gel Bands (1D/2D Coomassie stained)*
$180
$270
$360
  
 
Gel Bands-Predigested
$130
$220
$310
  
 
Gel Bands (silver stained)*
$225
$315
$465
  
 
Peptide Mapping
$200
$300
$400
  
 
Short-gel (unresolved protein mixture)*
$285
$395
$695
  
 
In-solution Digests (protein mixture)**
$285
$395
$695
  
 
Complex Digest-Predigested
$230
$345
$590
  
 
Replicate Runs (for quantitative proteomics)***
$155
$210
$420
  
  Enzymes other than Trypsin (e.g., PNGase F, Lys-C, Glu-C, Asp-N, Arg-C)
$60
$90
$120
  
 
1D SDS Gels (short or resolved, per gel)
$155
$210
$315
  
 
 
 
 
 
  
 
Intact Protein Analysis
 
 
 
  
 
Intact Mass Measurement
$155
$240
$315
  
 
 
 
 
 
  
 
  

What's Included:

*protein reduction/alkylation, in-gel digestion, extraction, LC-MS/MS, and informatics (database search for unmodified peptides)

**protein reduction/alkylation, in-solution or S-trap digestion, cleanup, LC-MS/MS, and informatics (database search for unmodified peptides)

***additional LC-MS/MS runs (ie 1-2 technical replicates)

Common Workflows:

• Protein Identification: LC-MS/MS (as listed above)
• Quantitative Proteomics (label-free or stable-isotope labeling methods): LC-MS/MS (above) + replicate runs 
   (above) + Staff Scientist/Director rate (as needed)
• Analysis of Post-translational Modifications (PTMs): LC-MS/MS (above) + Staff Scientist/Director rate (as  ccneeded)
• Quantitative Targeted Proteomics (peptide PRM or MRM): contact the Director for project proposal and cost

NOTE:  Studies needing protein/peptide labeling, protein/peptide fractionation, or immunodepletion of plasma proteins are charged at the Staff Scientist hourly rate (above).  Reagents, kits and other materials not included.

NOTE:  All reagents, standards, columns, kits ordered and received by the Mass Spectrometry Facility are charged at cost + 15% for internals and cost + 50% for externals.

 

  

The Mass Spectrometry Facility @ UMass Chan Medical School
Fuller Building
222 Maple Avenue
Shrewsbury, MA 01545
Inquiries