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Proteomics and Intact Protein Analysis

Rates effective January 1, 2018
    Rates (per hour)  
 

General Rates
 UMass
External
Academic
Corporate
  
 
Staff Scientist
 $50
$70
$120
  
 
Director
 $130
$175
$220
  
 
Instrument Time
 $150
$200
$300
  
       
    Rates (per sample)  
 

Proteomics (LC-MS/MS)
UMass
External
Academic
Corporate
  
 
Gel Bands (1D/2D Coomassie stained)*
$165
$225
$330
  
 
Gel Bands (silver stained)*
$220
$300
$440
  
 
Short-gel (unresolved protein mixture)*
$275
$375
$660
  
 
In-solution Digests (protein mixture)**
$275
$375
$660
  
 
In-solution Digests (single protein)**
 $165
$225
$330
  
 
Replicate Runs (for quantitative proteomics)***
$150
$200
$400
  
 
1D SDS Gels (short or resolved, per gel)
$150
$200
$300
  
 
  

What's Included:

*protein reduction/alkylation, in-gel digestion, extraction, LC-MS/MS, and informatics (database search for unmodified peptides)

**protein reduction/alkylation, in-solution or S-trap digestion, cleanup, LC-MS/MS, and informatics (database search for unmodified peptides)

***additional LC-MS/MS runs (ie 1-2 technical replicates)

  
 

 

Common Workflows:

  • Protein Identification: LC-MS/MS (as listed above)
  • Quantitative Proteomics (label-free or stable-isotope labeling methods): LC-MS/MS (above) + replicate runs (above) + Staff Scientist/Director rate as needed
  • Analysis of Post-translational Modifications (PTMs): LC-MS/MS (above) + Staff Scientist/Director rate as needed
  • Quantitative Targeted Proteomics (peptide PRM or MRM): contact the Director for project proposal and cost

NOTE: Studies needing protein/peptide labeling, protein/peptide fractionation, or immunodepletion of plasma proteins are charged at the Staff Scientist hourly rate (above). Reagents, kits and other materials not included. 

NOTE: All reagents, standards, columns, kits ordered and recieved by the Mass Spectrometry Facility are charged at cost + 15% for internals and cost + 50% for externals

  
   
Rates (per sample)
  
 

Intact Protein Analysis
UMass
External
Academic
Corporate
  
 
Intact Mass Measurement (proteins < 100 kDa) LC-MS
$100
$150
$200
  
 
Intact Mass Measurement (proteins > 100 kDa, mAbs) LC-MS
$150
$225
$300
  
 
Intact Mass Measurement (PTMs, ligand binding) LC-MS
$150
$225
$300
  
 
Intact Mass Measurement (proteins < 100 kDa) MALDI-TOF
$100
$150
$200
  

The Mass Spectrometry Facility @ UMass Chan Medical School
Fuller Building
222 Maple Avenue
Shrewsbury, MA 01545
Inquiries