RNAi Core Facility

The UMMS RNAi Core Facility houses the complete collections of human and mouse retroviral and lentiviral short hairpin RNA (shRNA) libraries from Open Biosystems, and distributes clones to UMass researchers (Amherst, Boston, Dartmouth, Lowell and Worcester campuses ONLY) at a reduced price.

The UMMS RNAi Core Facility currently has the following genome-wide libraries available for distribution: 

  • Human and mouse retroviral (pSM2c) shRNAmir libraries
    • Human releases 1.3-1.20 (~90,000 constructs)
    • Mouse releases 2.3-2.17 (~70,000 constructs)
    • Control clones (non-silencing, eGFP and luciferase)
  • Human and mouse lentiviral (pGIPZ) shRNAmir libraries
    • Human releases 6.1 to 6.33 (~153,600 constructs)
    • Mouse releases 7.1 to 7.21 (~100,800 constructs)
    • Control clones (non-silencing, GAPDH and empty vector)
  • Human and mouse lentiviral (TRC) shRNA libraries
    • Human (~85,000 constructs)
    • Mouse (~79,000 constructs)
    • Control clones (empty vector and eGFP)

Complete information regarding the retroviral and lentiviral shRNAmir libraries and the lentiviral TRC libraries can be found on the Open Biosystems website. In addition, please refer to the  Nature Methods paper and Nature Genetics Technical Report for information on the libraries.

 

Available Formats

shRNAs can be purchased as either single clones, pools (consisting of 5000 shRNAs/pool) for genome-wide screening, or sub-libraries in pool format.

Genome-wide pools

  • Retroviral (pSM2c) libraries
    • Human library: 13 pools
    • Mouse library: 13 pools
  • Lentiviral (pGIPZ) libraries
    • Human library: 32 pools
    • Mouse library: 21 pools
  • Lentiviral (TRC)  libraries
    • Human library: 22 pools
    • Mouse library: 24 pools

Sub-library pools

  • Lentiviral (TRC) libraries
    • Human kinase family: 781 genes (represented by 4518 shRNA constructs)
    • Human phosphatase family: 164 genes (represented by 817 shRNA constructs)
    • Human ubiquitin-ligase family: 616 genes (represented by 2833 shRNA constructs)
    • Mouse kinase family: 734 genes (represented by 3686 shRNA constructs)