Metabolic Profiling

Regulation of carbon metabolism is critical for M. tuberculosis to adapt to distinct host environments. To understand these adaptations, we use genetic approaches to identify critical genes and metabolomic profiling to understand the functions of the encoded enzymes and biochemical pathways.


Metabolic_profiling_picture1

Q-TOF LC-MS/MS for metabolomics

Metabolic_profiling_picture2

Critical biochemical pathways used for the catabolism of cholesterol were inferred via metabolite profiling (from Griffin et al. Chem Biol. 19:18-227. 2012.

Flux

Carbon fluxes are directly quantified by 13C tracing.