Services
Data Collection
- Cryo-EM imaging at low-dose condition and liquid nitrogen temperature
- High image quality, high throughput
- Automated cryo-ET data collection
- Automated single particle data collection
- Options for staff assist operation and remote control
Sample Preparation
- Prepare cryo-EM grids
Cryo Grid Screening
- Screen cryo grid conditions on auto-loader scope
- Screening will provide low mag atlas, medium mag montages for a few good meshes, and a few images from good ice areas
Nano-particle Morphology Studies
· Full morphology analysis for samples such as Lipid-based Nano-Particles (LNP) and AAV like particles.
· Three different magnifications, ~50+ images in total.
· Option for statistics analysis for size distribution and particle shapes.
Image Processing
- Preliminary data processing for aligning movie frames in fly during data collection
- 3D reconstruction for tomography data
- 3D reconstruction of single particle data
Structure Determination
· We now have bandwidth to take full projects. You send sample, we provide final structure.
Training
- Basic theory of electron microscopy
- Hands-on training of basic TEM operation using existing EM Core Facility
- Advanced training on TEM operation using SerialEM
- Hands-on training for operating Titan Krios and Talos-Arctica
- Negative stain sample preparation
- Cryo-EM sample preparation
For information on Training, please see Core Director.