Pacific Biosciences Core Enterprise
The Core operates a Pacific Biosciences RSII Instrument with the newest version of the run chemistry and SMRTportal data analysis. The service includes library construction (from submitted DNA or RNA) and sequencing. Data collection times available are 120, 240, or 360 minutes, with read lengths of up to 30kb. We also offer IsoSeq analysis and base modification detection. Multiplexing is available using either internal PacBio Barcoded Universal Primers (for amplicon libraries) or PacBio Barcoded Adapters. Please contact us about your project. We'd love to work with you!
- An explanation of Single-Molecule Real-Time Sequencing.
- Please email us to request a pricing list.
- To request the current version of the sequencing ticket click here.
- Sample preparation information and the FAQ are here.
Data and Bioinformatics
- UMMS and HHMI-NE users may register for the PacBio Portal where you will find analysis tools, open-source protocols, and other documentation:
- SMRTANALYSIS SOFTWARE is available for UMassMed users on GHPCC.
Go to /share/apps/smrtanalysis-2.0 for command-line access.
- The SMRT PIPE Manual is available at http://biotools.umassmed.edu/BioCore/nextgen/.
Samples can be left in the labeled fridges at these locations
for 11:00 AM pickup each business day:
Biotech2 outside Suite 207
LRB 6th floor mailroom
We are also available by appointment if you need to visit with us.
Our shipping address for off-campus customers is
Rose-Gordon Bldg. Rm. 141, 222 Maple Ave, Shrewsbury, MA, 01545.
We recommend eligible users get an account on the Green High Performance Computing Cluster for data delivery. See this WIKI for more information. To get an account, contact the UMassMed Help Desk at 856 - 8643, email them at firstname.lastname@example.org, or request access through this form.
Alternatives for non-UMass customers are to have the data uploaded to an outside server (using SFTP) or transferred to an external drive meeting our requirements and shipped overnight.
The Origin of the Haitian Cholera Outbreak Strain
Direct Detection and Sequencing of Damaged DNA Bases
Direct Detection of DNA Methylation During Single-Molecule, Real-Time Sequencing
Characterization of DNA methyltransferase specificities using single-molecule, real-time DNA sequencing
Validation of ITD mutations in FLT3 as a therapeutic target in human acute myeloid leukaemia
Going beyond five bases in DNA Sequencing