Pacific Biosciences Core Enterprise

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    The Core operates a Pacific Biosciences RSII Instrument with the newest version of the run chemistry and SMRTportal data analysis. Service includes library construction from submitted DNA or RNA and sequencing. Data collection times available are 120, 240, or 360 minutes, with read lengths of up to 30kb. We also offer IsoSeq analysis and base modification detection. Please contact us about your project; we'd love to work with you.

The Core has 3 drop off locations where samples can be left in the 
labeled fridge for 11:00 AM pickup each business day:
Biotech2 outside Suite 207
LRB 6th floor mailroom
ASC8-2058 mailroom
We are also available by appointment if you need to visit with us.
Our shipping address for off-campus customers is
Rose-Gordon Bldg. Rm. 141, 222 Maple Ave, Shrewsbury, MA, 01545.

    We recommend eligible users get an account on the Green High Performance Computing Cluster for data delivery. See this WIKI for more information. To get an account, contact the UMassMed Help Desk at 856 - 8643, email them at helpdesk@umassmed.edu, or request access through this form.

    Alternatives for non-UMass customers are to have the data uploaded to an outside server (using SFTP) or transferred to an external drive meeting our requirements and shipped overnight.

Publications:

The Origin of the Haitian Cholera Outbreak Strain
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3030187/

Direct Detection and Sequencing of Damaged DNA Bases
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3264494/

Direct Detection of DNA Methylation During Single-Molecule, Real-Time Sequencing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2879396/

Characterization of DNA methyltransferase specificities using single-molecule, real-time DNA sequencing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3287169/

Validation of ITD mutations in FLT3 as a therapeutic target in human acute myeloid leukaemia
http://www.ncbi.nlm.nih.gov/pubmed/22504184

Going beyond five bases in DNA Sequencing
http://www.ncbi.nlm.nih.gov/pubmed/22575758
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