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Deep Sequencing Core
(DSCL, MBCL, & PBCE)
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Deep Sequencing Core
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Sample Submission
Resources
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Deep Sequencing Core
Home
Services
Sample Submission
Resources
FAQ
Library Troubleshooting
Contact Information
COVID-19 Resources
UMassMed Deep Sequencing Core Labs - Illumina Sample Submission Ticket
DSCL Sample Submission Form
Investigator/Client Information
Your Name
*
Email
*
PI/Lab
*
PI's email
*
Phone Number
Mailing address
Account to be charged (speed type, account number, PO number, etc). 'On-file' will not be accepted - please enter a valid number or have your PI enter the correct Speed Type in the confirmation phase.
*
Sample Information
Are the sample(s) infectious or pathogenic to humans?
*
-- Select an option --
No
Yes
Describe the material(s) and any potential biohazards, if applicable
Number of samples in this group
*
Library adapters used
*
-- Select an option --
TruSeq DNA/RNA/Exome/PE
TruSeq Small RNA
TruSeq Stranded RNA
TruSeq Targeted Capture
Nextera
Chromium 10X Genomics
NEB
Takara/Clontech
NanoString
Custom (must be pre-approved by the Core)
Other 3rd-party kit or Illumina adapter
Adapter/kit version or description
Library Schematic (Please submit documentation on your library construction if applicable)
Multiplexing, Indexing, and Barcodes
i7 index length (How many bases should the i7 index read be? The standard lengths are between 8 and 12 bases. Indexes shorter than 6 bases should be discussed with the Core prior to submitting for the NovaSeq.)
i5 index length (How many bases should the i5 index read be? The standard lengths are between 8 and 12 bases. Indexes shorter than 6 bases should be discussed with the Core prior to submitting for the NovaSeq.)
Length & location of non-random bases (We need to know if there are any internal barcodes, UMIs, linkers, non-random, or homogenous sequences in the library. If so, please describe the length and position within the insert.)
Please upload the DSCL Sample Information List file containing your sample name(s) and information. The latest version of the excel sheet can be downloaded from our website at https://www.umassmed.edu/nemo/sample-submission/#tickets
*
Notes for the Core about your samples or library build
Sequence Analysis Run Type
Instrument requested
*
NovaSeq 6000
MiSeq
NovaSeq Options
NovaSeq Read Length
-- Select an option --
Single Read 100 bases
Paired End Read 50 bases
Paired End for Chromium 10X pipeline
Paired End Read 100 bases
Paired End Read 150 bases (full flowcell only)
Chromium 10X Read 1 Length (if applicable). The run must be <=138 bases total including index reads.
Chromium 10X Read 2 Length (if applicable). The run must be <=138 bases total including index reads.
phiX DNA control percentage *This addition is required for libraries with low sequence diversity/complexity (such as Chromium 10X) to ensure the base balance needed for optimal imaging. Please Note: Based on the information you provide, should we deem it necessary we will automatically add the appropriate % of PhiX DNA to your sample(s).
1% (NovaSeq standard)
5%
10%
15%
20%
25%
50%
MiSeq Options
MiSeq Read Length
-- Select an option --
Single Read 50 bases
Paired End Read 25 bases
Single Read 100 bases
Paired End Read 100 bases
Single Read 150 bases
Paired End Read 150 bases
Paired End Read 250 bases
Paired End Read 300 bases
Asymmetric Read
MiSeq Read 1 Length for Asymmetric Reads (if applicable) - must be at least 24 bases
MiSeq Read 2 Length for Asymmetric Reads (if applicable) - must be at least 24 bases
phiX DNA control percentage *This addition is required for libraries with low sequence diversity/complexity (such as Chromium 10X) to ensure the base balance needed for optimal imaging. Please Note: Based on the information you provide, should we deem it necessary we will automatically add the appropriate % of PhiX DNA to your sample(s).
1%
5%
10%
15%
20%
25%
50%
Data Delivery Information
Data Processing options **Note: If your data requires special processing, e.g. merging of files or saving machine files, please give us specific instructions at the time samples are submitted. There will be an additional comments field at the end of this form if needed.
*
-- Select an option --
Standard - Results will be in fastq files for Read1 and Read2; index reads are listed in the comment line
Demux - Fastq files for Read1 and Read2 are binned by identical index sequences, which are also listed in the comment line
Standard Individual Reads - Results will be in separate fastq files for Read1, Read2, i7, and i5
Demux Individual Reads - For each group/bin of identical indexes, results will be in separate fastq files for Read1, Read2, i7, and i5
If you have a Globus account, what is the email address assigned to it?
Do you want md5sum values?
Yes
No
Data transfer contact(s) (Name and email)
*
Data notification contact(s) (Name and email)
*
Payment Policy
Final Comments
Submit
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