TIME = 7 days
TIME = 13-18 days, depending upon drug marker.
TIME = 8 days
TIME = 10-12 days
Time from delivery of targeting vector to Core to delivery of DNA plates to the investigator for analysis is approximately 5-6 weeks, assuming no queue exists for targeting.
TIME = 20-25 days
Total time from delivery of targeting vector to Core to delivery of targeted clone(s) either to investigator or to Transgenic Core is approximately 8-9 weeks, plus the time it takes investigator to analyze clones in 96-well format (typically 2-3 weeks) and to confirm clone is correctly targeted at end of procedure (typically 2 weeks). Thus, the total time it takes to generate a targeted ES clone, assuming no queue exists for targeting and all goes well, is 12-14 weeks. Please note there is little the Gene Targeting Core can do to hasten this time frame- as it is mostly a function of how long it takes cells to undergo drug selection and how long it takes an individual clone to grow sufficiently for analysis or freeze down.
Additional modifications (Cre addition and reselection, etc) will require additional time, and may require a third screening of clones by the investigator to confirm proper targeting of the allele.
TIME = 15-30 days, depending upon the procedure.
Regarding the queue: The targeting Core is able to initiate 3 new targeting experiments each week. Typically, the queue is 0-1 week, and has never been more than 3 weeks. While the Core operates on a “first come- first served” basis, we do reserve the right to stagger individual investigator’s targeting experiments so that no single lab dominates use of the Core to the exclusion of other groups (example: a lab turning in 6 constructs simultaneously)