GETTING STARTED
Complete our MPCF form which is sent to Jim Crandall, Core Director. Dr. Crandall will contact you to set up a meeting to discuss your project.
At the initial meeting, issues such as who does what, staining protocols, timeframe(s), desired outcomes, and a jointly agreed upon plan of action will be discussed.
Be sure to bring tissue, or blocks or slides to the meeting.
All projects will be managed on a first come-first served basis.
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Routine immunoperoxidase (ABC-POD-DAB) is the most widely used combination used by this facility.
For new antibodies, the investigator should be prepared to provide: 1) antibody, 2) product information, 3) publications documenting antibody's use, 4) positive and negative control samples (cells or tissues), and any other information to assist with development.
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Trouble-shooting technical difficulties and problem solving are performed as collaborative efforts with the investigator and their staff. Limited training for investigators and their staff is provided for tissue preparation, microtomy and staining procedures.
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SERVICES
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Tissue Fixation
Small rodent vascular perfusion
(embryos and adults), tissue
immersion, cryoimmersion
Microtomy
Frozen (sledge or cryostat),
Vibratome, Paraffin
Cell (Nissl stains)
Immunohistochemistry
Free floating sections or on slide ABC-POD,
Fluorochromes, double and triple label
In Situ Hybridization
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EVALUATION
Upon completion of the first pilot experiment, slides will be returned to the investigator and evaluated together with the MPFC director. Feedback as to protocol adjustments will be developed and adjustments made if necessary before proceeding with the remainder of the tissue samples.