Biomarker Development - Selected Publications

1. Chen X, Dou S, Liu G, Liu X, Wang Y, Chen L, Rusckowski M, and Hnatowich DJ. Synthesis and in vitro characterization of a dendrimer-MORF conjugate for amplification pretargeting. Bioconjugate Chem. 2008; 19:1518-1525.

2. Chen L, Dou S, Liu G, Liu X, Wang Y, Hnatowich DJ, Rusckowski M. A new peptide binds to TAG-72 cancer marker identified by phage display. Cancer Letters, in press 2008.

3. Liu G, Dou S, Rusckowski M, Hnatowich DJ. An experimental and theoretical evaluation of the influence of pretargeting antibody on the tumor accumulation of effector. Mol Cancer Ther. 2008;7: 1025-1032.

4. Kleine LG, Solano M, Rusckowski M, Hunt KE, Johnson KL, Kirker-Head CA. Evaluation of technetium 99mTc -labeled biotin for scintigraphic detection of soft tissue inflammation in horses. American Journal of Veterinary Research. 2008;69:639-646.

5. Rusckowski M, Gupta S, Liu, G, Dou S, Hnatowich DJ. Investigation of four99mTc-labeled bacteriophages for infection specific imaging. Nucl Med and Biol. 2008;35:433-440.

6. Liu G, Dou S, Pretorius PH, Liu X, Rusckowski M, Hnatowich DJ. Pretargeting CWR22 prostate tumor in mice with MORF-B72.3 antibody and radiolabeled cMORF. Eur J Nucl Med Mol Imaging. 2008;35:272-280.

7. Devy L, Rabbani SA, Stochl M, Rusckowski M, Mackir I, Naa L, Toews M, van Gool R, Chen J, Ley A, Ladner RC, Dransfield DT, Henderikx. PEGylated DX-1000: Pharmacokinetics and antineoplastic activity of a specific plasmin inhibitor. Neoplasia. 2007; 9:927-937.

8. Zhang S, Liu G, Liu X, Yin D, Dou S, He J, Rusckowski M, Hnatowich DJ. Comparison of several linear fluorophore – and quencher – conjugated oligomer duplexes for stability, fluorescence quenching and kinetics in vitro and in vivo in mice. Bioconjug Chem. 2007;18:1170-1175.

9. Rusckowski M, Gupta S, Liu G, Dou S, Hnatowich DJ. Evidence of specificity of radiolabeled phage display peptides for the TAG-72 antigen. Cancer Biotherapy and Radiopharmaceuticals. 2007; 22:564-572.

10. Liu X, Wang Y, Nakamura K, Liu G, Dou S, Kubo A, Rusckowski M, Hnatowich DJ. Optical antisense imaging of tumor with fluorescent DNA duplexes. Bioconjug. Chem. 2007;18:1905-1911.

11. He J, Liu G, Dou S, Gupta S, Rusckowski M, Hnatowich DJ. An improved method for covalently conjugating morpholino oligomers to antitumor antibodies. Bioconjug Chem. 2007;18:983-988.

12. Liu G, Dou S, Yin D, Squires S, Liu X, Wang Y, Rusckowski M, Hnatowich DJ. A novel pretargeting method for measuring antibody internalization in tumor cells. Cancer Biotherapy and Radiopharmaceuticals. 2007;22:33-39.

13. He J, Rusckowski M, Wang Y, Dou S, Liu X, Zhang S, Liu G, Hnatowich DJ. Optical pretargeting of tumor with fluorescent MORF oligomers. Mol Imaging Biol. 2007; 9:17-23.

14. Wang Y, Liu X, Liu G, Zhang YM, Rusckowski M, Hnatowich, DJ. Nonspecific cellular accumulation of 99mTc-labeled DNA's in culture is influenced by their guanine content. Nucl Med Biol. 2007;34: 47-54.

15. Liu G, Dou S, He J, Liu X, Rusckowski M, Hnatowich DJ. Predicting the biodistribution of radiolabeled cMORF effector in MORF-pretargeted mice. Eur J Nucl Med Mol Imaging. 2007;34:237-246.

16. Liu X, Nakamura K, Wang Y, Zhang S, He J, Liu G, Dou S, Kubo A, Rusckowski M, Hnatowich DJ. Improved delivery in cell culture of radiolabeled antisense DNAs by duplex formation. Molecular Imaging and Biology. 2006;8:278-283.

17. Liu G, Dou S, Mardirossian G, He J, Zhang S, Liu X, Rusckowski M, Hnatowich DJ. Successful radiotherapy of tumor in pretargeted mice by 188Re radiolabeled phosphorodiamidate morpholino oligomer, a synthetic DNA analogue. Clin Cancer Res. 2006;12:4958-4964.

18. Liu G, Dou S, He J, Yin D, Gupta S, Zhang S, Wang Yi, Rusckowski M, Hnatowich DJ. Radiolabeling of MAG3-morpholino oligomers with 188Re at high labeling efficiency and specific radioactivity for tumor pretargeting. Applied Radiation and Isotopes. 2006;64:971-978.

19. Zhang Y-M, Tung CH, He J, Liu N, Yanachkov I, Liu G, Rusckowski M, Vanderheyden J-L. Construction of a novel chimera consisting of a chelator-containing Tat peptide conjugated to a morpholino antisense oligomer for technetium-99m labeling and accelerating cellular kinetics. Nucl Med Biol, 2006; 33:263-269.

20. Liu X, Kayoko N, Wang Y, Wang Y, Liu G, He J, Ding H, Lu P, Rusckowski M, Kubo A, Hnatowich DJ. Initial mechanistic studies of antisense targeting in cells. J Nucl Med. 2006; 47:360-368.

21. Liu G, He J, Dou S, Gupta S, Rusckowski M, Hnatowich DJ. Further investigations of morpholino pretargeting in mice-- establishing quantitative relations in tumor. Eur J Nucl Med Mol Imaging. 2005; 32: 1115-23.

22. He J, Liu G, Vanderheyden J-L, Dou S, Rusckowski M, Hnatowich DJ. Affinity enhancement bivalent morpholino for pretargeting: Initial evidence by surface plasmon resonance. Bioconjugate Chem. 2005; 16:338-345.

23. Liu G, Dou S, He J, Vanderheyden J-L, Rusckowski M, Hnatowich DJ. Preparation and properties of 99mTc(CO)3+ labeled N,N-bis(2-pyridylmethyl)-4-aminobutyric acid. Bioconjugate Chem, 2004;15:1441-1446.

24. Nakamura K, Fan C, Liu G, Gupta S, He J, Dou S, Kubo A, Rusckowski M, Hnatowich DJ. Evidence of antisense tumor targeting in mice. Bioconjugate Chem, 2004;15: 1475-1480.

25. Zhang YM, He J, Liu G, Vanderhayden JL, Gupta S, Rusckowski M, Hnatowich D. Initial observation of 99mTc-labeled locked nucleic acids for antisense targeting. Nucl Med Communications 2004;25:1118-1123.

26. Rusckowski M, Gupta S, Liu G, Dou S, DJ Hnatowich. Investigations of a 99mTc-bacteriophage as a potential infection specific imaging agent. J Nucl Med. 2004; 45:1201-1208.

27. Liu G, He J, Dou S, Gupta S, Vanderheyden J-L, Zhang Y, Rusckowski M, Hnatowich DJ. Pretargeting in tumored mice with radiolabeled morpholino oligomer showing low kidney uptake. Eur J of Nucl Med and Mol Imaging, 2004;31:417-424.

28. He J, Liu C, Vanderheyden J-L, Liu G, Dou S, Rusckowski M, D J Hnatowich. Radiolabeling morpholinos with 188Re carbonyl provides improved in vitro and in vivo stability to re-oxidation. Nucl Med Commun. 2004; 25:731-736.

29. He J, Liu G, Gupta S, Zhang Y-M, Rusckowski M, D J Hnatowich. Amplification targeting: A Modified pretargeting approach with potential for signal amplification- proof of a concept. J Nucl Med 2004;45:1087-1095.

30. He J, Liu G, Zhang S, Rusckowski M, D J Hnatowich. Pharmacokinetics in mice of four oligomer-conjugated polymers for amplification targeting. Can Biotherapy and Radiopharmaceuticals. 2003;18:941-947

31. He J, Liu G, Zhang S, Vanderheyden J-L, Liu N, Liu C, Zhang Y, Gupta S,Rusckowski M, Hnatowich DJ. A comparison of in vitro and in vivo stability in mice of two morpholino duplexes differing in chain length. Bioconjug. Chem, 2003;14:1018-1023

32. Zhang YM, Liu CB, Liu N, Flores GF, He J, Rusckowski M, Hnatowich DJ. Electrostatic binding with Tat and other cationic peptides increases cell accumulation of 99mTc-antisense DNAs without entrapment. Molecular Imaging and Biology, 2003;5:240-247.

33. Ferro-Flores G, Arteaga de Murphy C, Pedraza-Lopez M, Melendez-Alafort L, Zhang Y-M, Rusckowski M, D J Hnatowich. In vitro and in vivo assessment of 99mTc-UBI specificity for bacteria. Nucl Med and Biol. 2003;30:597-603.

34. Su Zi-Fen, He J, Rusckowski M, Hnatowich DJ. In vitro cell studies of technetium-99m labeled RGD-HYNIC peptide, a comparison of tricine and EDDA as co-ligand. Nuclear Medicine and Biology, 2003; 30: 141-149.

35. Liu G, Liu C, Zhang S, He J, Liu N, Gupta S, Rusckowski M, Hnatowich DJ. Investigations of Technetium-99m Morpholino Pretargeting in Mice. Nuclear Medicine Communications 2003; 24:697-705.

36. Liu CB, Liu GZ, Liu N, Zhang YM, He J, Rusckowski M, Hnatowich DJ. Radiolabeling morpholinos with 90Y, 111In, 188Re and 99mTc. Nuclear Medicine and Biology 2003; 30:207-214.

37. Liu G, He J, Zhang S, Liu C, Rusckowski M, Hnatowich DJ. Cytosine Residues Influence Kidney Accumulations of 99mTc-Labeled Morpholino Oligomers. Antisense & Nucleic Acid Drug Development. 2002;12:393-398.

38. Liu G, Zhang S, He J, Liu N, Gupta S, Rusckowski M, Hnatowich DJ. The influence of chain length and base sequence on the pharmacokinetic behavior of99mTc-morpholinos in mice. Quarterly J Nucl Med 2002; 46: 233-243.

39. Liu G., Zhang S, He J, Zhu Z, Rusckowski M, Hnatowich D. Improving the labeling of S-Acetyl NHS-MAG3-conjugated morpholino oligomers. Bioconjugate Chem. 2002;13:893-897.

40. Liu G, Mang’era K, Liu N, Gupta S, Rusckowski M, Hnatowich DJ. Tumor pretargeting in mice using 99mTc labeled morpholinos, a DNA analog. J Nucl Med. 2002;43:384-391.

41. Su Z., Zhu Z., Liu G., Gupta S., Liu C., Zhang Y., Liu N., Rusckowski M., Hnatowich DJ. In vitro and in vivo evaluation of a technetium-99m labeled RGD peptide as a specific marker of avb3 integrins for tumor imaging. Bioconjugate Chem. 2002; 13:561-570.

42. Liu G, Wescott C, Sato A, Wang Yi, Liu N, Zhang Y-M, Rusckowski M, Hnatowich DJ. Nitriles form mixed-coligand complexes with 99mTc-HYNIC-peptide. Nucl Med and Biol. 2002;29:107-113.

43. Zhang Y-M, Rusckowski M, Liu N, Liu C, Hnatowich. Cationic Liposomes Enhance Cellular/Nuclear Localization of 99mTc-Antisense Oligonucleotides in Target Tumor Cells. Cancer Biotherapy and Radiopharmaceuticals. 2001; 16:411-419.

44. Rusckowski M, Qu T, Gupta S, Ley A, Hnatowich D. A comparison in Monkeys of99mTc labeled to a peptide by 4 methods. J Nucl Med. 2001;42:1870-1877.

45. Mang’era K, Liu G, Wang Y, Zhang Y-M, Liu N, Gupta S, Rusckowski M,Hnatowich D. Initial Investigation of 99mTc-Labeled Morpholinos for Radiopharmaceuticals Applications. Eur J Nucl Med. 2001;28:1682-1689.

46. Zhu Z-H, Wang Y, Rusckowski M, Hnatowich DJ. A novel and simplified route to the synthesis of N3S chelators for 99mTc labeling. Nuclear Medicine and Biology. 2001;28:703-708.

47. Wang Y, Chang F, Zhang Y-M, Liu N, Liu G, Gupta S, Rusckowski M, Hnatowich DJ. Pretargeting with amplification using polymeric peptide nucleic acid. Bioconjugate Chemistry. 2001; 12:807-816.

48.Zhang Y, Wang Y, Liu N, Zhu Z-H, Rusckowski M, Hnatowich DJ. In vitro investigations of tumor targeting with Tc-99m labeled antisense DNA. J. Nucl Med. 2001;42:660-1669.

49.Qu T, Wang Y, Zhu Z, Rusckowski M, Hnatowich DJ. Different chelators and different peptides together influence their in vitro and mouse in vivo properties of99mTc. Nucl Med Comm. 2001;22:203-215.

50. Zhang Y, Liu N, Zhu Z-H, Rusckowski M, Hnatowich DJ. Influence of different chelators (HYNIC, MAG3, and DTPA) on tumor cell accumulation and mouse biodistribution of 99m-Tc labeled to antisense DNA. Eur J Nucl Med. 2000; 27:1700-1707.

51. Rusckowski M, Qu T, Roskey A, Agrawal S. Biodistribution and metabolism of a mixed backbone oligonucleotide (GEM 231) following single and multiple dose administration in mice. Antisense & Nucleic acid Drug Dev. 2000; 10: 333-345.

52. Rusckowski M, Qu T, Pullman J, Marcel R, Ley AC, Ladner RC, Hnatowich DJ. Inflammation/infection imaging with a 99m-Tc-neutrophil elastase inhibitor in monkeys. J Nucl Med. 2000; 41:363-374.

53. Chang F, Qu T, Rusckowski M, Hnatowich DJ. NHS-MAS3 - A Bifunctional chelator alternative to NHS-MAG3. Applied Radiation and Isotopes. 1999;50:723-732.

54. Fogarasi M, Pullman J, Winnard P, Hnatowich DJ, Rusckowski M. Pretargeting of bacterial endocarditis in rats with streptavidin and 111In-labeled biotin. J Nucl Med. 1999;40: 484-490.

55. Jiang Z, Kandimalla E, Zhao Q, Shen L, DeLuca A, Normano N, Rusckowski M, Agrawal S. Pseudo-Cyclic Oligonucleotides: In Vitro and In Vivo Properties. BioOrganic and Medicinal Chemistry. 1999;7: 2727-2735.

56. Hnatowich DJ, Chang F, Qu T, Rusckowski M. Techneium-99m labeled peptides-an investigation of multiple HPLC peaks. Applied Radiation and Isotopes. 1998;00:1-11.

57. Hnatowich DJ, Qu T, Chang F, Ley AC, Ladner RC, Rusckowski M. Labeling Peptides with 99mTc using an NHS-MAG3 Bifuctional Chelator. J. Nucl Med. 1998;39:56-64.

58. Rusckowski M, Qu T, Chang F, Hnatowich DJ. Pretargeting Using Peptide Nucleic Acid (PNA). Cancer. 1997;80:2699-2705.

59. Winnard P, Rusckowski M, Mardirossian G, Hnatowich DJ. Preparation and use of NHS-MAG3 for Techetium-99m labeling of DNA. Nuc Med and Biol. 1997;24:425-432.

60. Chang F, Rusckowski M, Qu T, Hnatowich DJ. Early Results in the Irrational Design of New Bifunctional Chelators. Cancer. 1997;80:2347-2353.

61. Rusckowski M, Qu T, Chang F, Hnatowich DJ. Technetium-99m labeled epidermal growth factor-tumor imaging in mice. J Peptide Research. 1997;50:393-401.

62. Mardirossian G, Lei K, Rusckowski M, Chang F, Qu T, Egholm M, Hnatowich DJ. In vivo hybridization of technetium-99m-labeled peptide nucleic acid (PNA). J Nucl Med. 1997;38:907-913.

63. Hnatowich DJ, Chang F, Lei K, Qu T, Rusckowski M. The Influence of Temperature and Alkaline pH on the Labeling of Free and Conjugated MAG3 with Technetium-99m. Appl. Radiat and Isot. 1997;48:587-594.

64. Rusckowski M, Fogarasi M, Fritz B, Hnatowich DJ. Effect of endogenous biotin on applications of streptavidin and biotin in mice. Nucl Med and Biol. 1997;24:263-268.