To initiate a project, submit a sample, or schedule instrument time, please follow the WORKFLOW described (on right).
All projects and services are subject to approval by the Facility Director. Most new projects require a consultation prior to initiation of work. Consultations include advice on the best experimental approach, timing of the experiment, sample preparation and charges. Samples are accepted only after the project has been approved and assigned to a Facility staff member.
Rates. Our rate sheet captures the per sample cost or hourly rate for most of our services. In general, costs for most proteomics projects can be determined from the rate sheet. However, most small molecule projects are quotated by proposal and initiate following mutual acceptance.
The Facility will not accept BIOHAZARDOUS and RADIOLABELED samples.
RG-3 or select agents (http://www.selectagents.gov) WILL NOT BE ACCEPTED unless inactivated by a process approved by an Institutional Biosafety Committee (IBC).
Sample preparation by SDS PAGE should be performed with a dedicated gel electrophoresis apparatus for protein analysis. All glassware and reagents should be freshly prepared and handled with gloves at all times. Gel bands can be stained with Coomassie Blue or colloidal variants. For silver staining use a mass spectrometry compatible kit (e.g. Sigma ProteoSilver, or BioRad Silver Stain Plus). It is extremely important to remember that when preparing low abundance proteins that precautions must be taken to prevent keratin contamination. Wear gloves and excise bands of interest in a dust free environment. Excised gel bands should be thoroughly washed with Milli-Q grade water and placed in 1.5 ml microcentrifuge tubes (pre-rinsed) with a little water to keep them moist. Gel bands should be stored at 4oC prior to shipping. For samples submitted for intact molecular weight analysis please consult with a staff member on the chemical makeup of the solution for compatibility with mass spectrometry.
Gel bands can be shipped via an overnight courier at ambient temperature. Samples should be placed in a secondary container to prevent tube breakage during shipment. Solution phase samples that are not completely dried down should be shipped on dry ice. Please send notification via email prior to shipping so that a staff member can take receipt of your samples.
For compliance on packaging and transport of hazardous substances see IATA (http://www.iata.org/whatwedo/cargo/dangerous_goods/Pages/infectious_substances.aspx) and DOT (http://www.phmsa.dot.gov/hazmat) for additional guidance.