•Small Molecule Quantitation
Much of what we do can be broadly grouped accordingly. Below is a list of technology approaches and application areas. If you don't find what you are looking for, please inquire within.
Electrospray ionization (ESI) liquid chromatography tandem mass spectrometry (LC-MS/MS)
Comprehensive peptide identification of simple and complex protein digests from gel bands (e.g. Coomassie blue and silver stained), immunoprecipitation (IP), and whole-cell/secreted/tissue digests by shotgun proteomics. Approaches for maximizing proteomic coverage, peptide quantitation, and de novo peptide sequencing offered. Identification and localization of post-translational modifications, chemically cross linked peptides, protein-drug adducts and disulfide mapping. Whole protein analysis is also available. LC-MS profiling, exact mass measurement, structure elucidation and quantitation of small molecules (drugs, contaminants), lipids, glycolipids, and metabolites. Applications to drug metabolism, pharmacokinetics, lipidomics, and metabolomics.
Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry
Peptide identification of simple protein digests from gel bands/2D spots (e.g. Coomassie blue and silver stained) and purified proteins. De novo peptide sequencing and In-Source-Decay (ISD) N-terminal/C-terminal sequence verification of purified recombinant proteins. Identification and localization of chemical and post-translational modifications. Analysis of whole proteins, synthetic polymers, carbohydrates, and glycolipids.
Protein and peptide fractionation
Fractionation technologies include one-dimensional gel electrophoresis, immuno-depletion of abundant proteins from biological fluids, and semi-preparative liquid chromatography (e.g. strong cation exchange, reverse-phase, normal phase).