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Craig Mello, Ph.D.Academic Role: Professor
Faculty Appointment(s) In:
Other Affiliation(s):
Figure LegendA 28-cell stage C. elegans embryo stained with Dapi to reveal nuclei (blue) and with a monoclonal antibody raised against the PIE-1 protein (red). The PIE-1 protein is observed in the fertilized egg and is sequentially restricted to the germline stem cell after each division in the embryo. PIE-1 functions to prevent the germ cell from differentiating in response to somatic determinants and signals which are actively patterning the embryo during this time. Understanding the localization and function of PIE-1 is a major goal of research in our laboratory. 
Figure LegendA 2-cell C. elegans embryo divides to produce 4-cells in this series of images. On the left Nomarski microscopy reveals the grainy yolk droplets in the cytoplasm and the smooth circular nuclei in the center of the cells. On the right, the same embryos are imaged to visualize GFP (Green Fluorescent Protein) which has been tagged onto the PIE-1 protein. During this sequence of images, the PIE-1::GFP fusion protein first becomes nuclear, then during mitosis associates with the centrosome (middle panels), finally after cell division the protein re-enters the nucleus. PIE-1 functions to prevent the germ cell from differentiating in response to somatic determinants and signals which are actively patterning the embryo during this time. Understanding the localization and function of PIE-1 is a major goal of research in our laboratory. We are using this GFP construct to follow PIE-1 protein in mutant backgrounds and to determine what parts of the PIE-1 protein control its embryonic and subcellular localization.
Office: Biotech Two, Suite 219
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373 Plantation Street Worcester, MA 01605