Section: Rotations

Cheryl Scheid, Ph.D.

Academic Role: Professor

Faculty Appointment(s) In:
   Surgery

Potential Rotation Projects

Project #1: Studies on gene expression using microarray analysis. Studies are in progress to monitor oxalate-induced changes in gene expression in cultured renal epithelial cells. Parallel studies will soon be initiated on human renal tissue from patients with kidney stone disease. Such studies are a collaborative effort with surgeons caring for stone patients and should provide important new information as to the underlying cellular changes that accompany stone formation in vivo.

Project #2: Detection of oxalate induced activation of caspase activation using GFP-FRET analysis. We have obtained a probe to monitor caspase activity using FRET (fluorescence resonance energy transfer). MDCK cells will be transfected with this probe and caspase activity will be monitored in individual cells after oxalate exposure. Such studies will provide a more robust assessment of the time course for oxalate actions on renal epithelial cells to better understand oxalate-induced changes in cell viability that may be important in the progression of kidney stone disease.

Office: S1-340
Phone: 508-856-1301
Fax: 508-856-8181
E-mail: Cheryl.Scheid.Forward@umassmed.edu
Keywords: Pathology, Clinical Research, Gene Expression, Signal Transduction

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