Cheryl Scheid, Ph.D.

Academic Role: Professor

Faculty Appointment(s) In:
   Surgery

Renal epithelial cell biology

1. Understanding cellular processes within the kidney that contribute to kidney stone formation , specifically those processes that are affected by oxalate, a major constituent of kidney stones.
2. Assessing the manner by which oxidant stress affects renal epithelial cell function, particularly pathways by which oxidants regulate gene expression, growth and viability of renal epithelial cells.

Other interests: Renal cellular mechanisms leading to polycystic kidney disease.

Recent Publications

Jonassen JA,  Kohjimoto Y, Scheid CR, Schmidt M. Oxalate toxicity in renal cells, Urol Res, In Press, 2005.

Scheid CR, Cao L-C, Honeyman T and Jonassen JA.  How elevated oxalate can promote kidney stone disease: changes at the surface and in the cytosol of renal cells that promote crystal adherence and growth.  Frontiers  Biosci.  9:787-808, 2004

Jonassen JA, Cao L-C, Honeyman T and Scheid CR. Intracellular events in the initiation of calcium oxalate stones.  Nephron Exp Nephrol  98:e61-64, 2004.

Cao LC, Honeyman TW, Jonassen J, Ying H, Cooney R, Kennington L and Scheid, CR. Mitochondrial dysfunction may be the primary event in oxalate toxicity.  Kidney Int  66:1890-1900, 2004.

Jonassen JA, Cao L-C, Honeyman TW and Scheid CR. Mechanisms mediating oxalate-induced alterations in renal cell functions.  Crit Rev Eukaryot Gene Exp 13:55-72, 2003.

Cao L-C, Jonassen J, Honeyman TW and Scheid CR.  Oxalate-induced redistribution of renal phosphatidylserine in renal epithelial cells.  Am J Nephrol  21:69-71, 2001

Scheid C, Honeyman T, Kohjimoto Y, Cao L-C, Jonassen J. Oxalate-induced changes in renal epithelial cell function: role in stone disease.  Molec Urol   4:371-381, 2000.

Miller C, Kennington, L, Cooney R, Honeyman T, Kohjimoto Y, Cao L-C, Pullman J, Jonassen J, and Scheid CR. Oxalate toxicity in renal epithelial cells: characteristics of apoptosis and necrosis. Toxicol Appl Pharm 162:132-141, 2000

Kohjimoto Y, Jonassen J, Scheid CR and Honeyman, T. Phospholipase A2 mediates immediate early genes in cultured renal epithelial cells: possible role of lysophospholipid.  Kidney Int  50:638-646, 2000.

Cao L-C, Honeyman TW, Jonassen J and Scheid CR. Oxalate-induced ceramide accumulation in Madin-Darby canine kidney and LLC-PK1 cells.   Kidney Int 57:2403-2411, 2000. 

Jonassen JA, Cooney R, Kennington L, Gravel K, Honeyman T and Scheid CR. Oxalate-induced changes in the viability and growth of human renal epithelial cells.  J Amer Soc Nephrol  10:S446-S451, 1999. 

Kohjimoto Y, Jonassen J, Scheid CR and Honeyman T.  Role of phospholipase A2 in the cytotoxic effects of oxalate in cultured renal epithelial cells.  Kidney Int  56:1432-1441, 1999.

Potential Rotation Projects

Project #1: Studies on gene expression using microarray analysis. Studies are in progress to monitor oxalate-induced changes in gene expression in cultured renal epithelial cells. Parallel studies will soon be initiated on human renal tissue from patients with kidney stone disease. Such studies are a collaborative effort with surgeons caring for stone patients and should provide important new information as to the underlying cellular changes that accompany stone formation in vivo.

Project #2: Detection of oxalate induced activation of caspase activation using GFP-FRET analysis. We have obtained a probe to monitor caspase activity using FRET (fluorescence resonance energy transfer). MDCK cells will be transfected with this probe and caspase activity will be monitored in individual cells after oxalate exposure. Such studies will provide a more robust assessment of the time course for oxalate actions on renal epithelial cells to better understand oxalate-induced changes in cell viability that may be important in the progression of kidney stone disease.

Academic Background

1970      B.S.    College of William and Mary
1972      M.A.    Boston University
1976      Ph.D.   Boston University

Vice Chancellor of Faculty Administration

Interim Provost

Office: S1-340
Phone: 508-856-1301
Fax: 508-856-8181
E-mail: Cheryl.Scheid.Forward@umassmed.edu
Keywords: Pathology, Clinical Research, Gene Expression, Signal Transduction

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