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Job Dekker, Ph.D.Academic Role: Associate Professor
Faculty Appointment(s) In:
Other Affiliation(s):
Figures
Figure 1. (a) Genes (blue rectangles) and regulatory elements (red circles) are linearly organized along chromosomes (top), but as a result of specific interactions between elements (indicated by arrows, both in cis and in trans) a complex three-dimensional network is formed inside the cell (bottom). (b) Schematic representation of the 3C assay. Chromatin is cross-linked, digested with a restriction enzyme and then ligated. Specific ligation products can be detected by PCR. [Figure from Dekker (2006), Nature Methods, 3(1): 17-21].
Figure 2. Schematic representation of the human beta-globin locus. Activation of the g-globin genes in K562 cells involves interactions between the LCR and the activated genes resulting in a large (~40 kb) chromatin loop. The LCR also interacts with an element located downstream of the locus (3’HS1).
Figure 3. Spatial organization of yeast chromosome III (~ 320 kb) as determined by Chromosome Conformation Capture (3C). 3C was applied to determine the frequency with which several loci along the chromosome interact. Interaction frequencies were then used to model the average spatial organization of the chromosome. Interactions between the telomeres result in the formation of a ring-like structure with a diameter of around 300 nm.
Office: LRB 519
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364 Plantation Street Worcester, MA 01605