Deep Sequencing Core Facility

The HiSeq2000's are completing validation trials, and we are accepting samples for the queue. Not all Illumina primers used for previously submitted libraries will work on the HiSeq platform. The New Sample Submission Ticket should be used for all DeepSeq samples, including HiSeq. For a pricing list or other HiSeq questions, please email us.

There is an updated list of sequences for Illumina library construction. If you are UMass investigator and wish to obtain a copy, please send an email to NEMO@umassmed.edu and request "the new oligo list".

A list of frequently asked questions and some answers are here
Puzzled about Multiplexing and Barcoding? Click Here.

    NOTE: It is VERY IMPORTANT that we receive the correct information about the primers and adapters used to build your library! Not all library types will run on the HiSeq instruments or can be read in both directions. If you are using the Illumina internal barcodes in the TruSeq adapaters, you must add a multiplex read step to detect your barcodes. There is more information in the FAQ.

If you would like to consult with an applications specialist, please email us to get on the schedule.

Link to BioTools for Next Gen Sequencing Documents click here
Information about NextGen BioInformatics Tools on HPCC click here

For information about the SOLiD4 and Color-Space sequencing click here

New Tool for Data Quality Score Viewing FASTQC

Payment Policy:  Processing and analyzing a sample requires time and reagents. Payment for these services is the responsibility of the user submitting the sample. In the event of a reagent or equipment failure, the samples will be rerun at the next possible opportunity at no additional charge.

WEATHER ALERT ~ ~ ~ In case of extreme and/or inconvenient weather, the machines will still be running but we may not be here for sample login. Please email us to arrange drop off if the weather is questionable. 

NEMO@umassmed.edu