October 2008

Dear Deep Sequencers,

Nemo's "extreme makeover" has been completed ! Thank you for your patience. We are now able to process samples faster, collect more data for you, .... AND .... offer Paired-End Reads!!!! We will continue to perform analyses on Chromatin IP, Genomic DNA, cDNA, Expression TAG and BarCoded samples, and small RNA libraries as well.

We have posted the official Illumina versions of the protocols on the services page (see link in the left nav column). FAQs and Core Lab versions of these protocols are being posted as they become available. Additionally, we welcome any user-submitted information (please send it to Nemo at the email below).

There is article about BarCodes and indexing in the recent issue (Oct 08) of Nature Methods ( available online ). Also, the January 2008 issue of Nature Methods (vol 5) has several useful articles about next generation sequencing (available online).

If you are preparing samples for submission, please fill out one of the Analysis Order Forms for each sample. Since samples may be run at different times, we need a tracking sheet for each one please.

The Paired-End sequencing (PE) uses a different second PCR primer which incorporates a second sequencing priming site. Since the reagents for the expanded data collection are more expensive we must raise pricing slightly. For information about the new primers and a revised UMassMed pricing list, please send us an email at nemo@umassmed.edu 

To join the email list please send us a note at   nemo@umassmed.edu 

 Please stay tuned for updates.

Thank you,

~ Nemo and Crew 

For a letter of support for a research grant click here.