Section: Rotations

Kenan Murphy, Ph.D.

Academic Role: Assistant Professor

Faculty Appointment(s) In:
   Molecular Genetics and Microbiology

Other Affiliation(s):
   Bacterial Genetics and Pathogenesis

Rotation Projects

1. Study the interaction of the ssDNA annealing protein Bet and its cognate exonuclease with dsDNA ends.  Compare wild type Bet with one or mutant Bet proteins.  Project involves a protein isolation, protein-DNA binding assays, and basic molecular biological techniques.
   
   
2. Electroporate linearized plasmids into E. coli wild type and recN mutants and follow the repair of the dsDNA ends by the emergence of  drug resistant colonies.  Determine the genetic requirements for efficient RecN-promoted repair. Project involves electroporation experiments, basic bacterial plating experiments and molecular biological techniques.
   
   
3. Help develop recombineering technology for Pseudomonas aeruginosa by the electroporation of PCR substrates into electrocompetent cells. Project involves electroporation experiments, basic bacterial plating experiments and molecular biological techniques.

Office: S6-137
Phone: 508-856-6042
E-mail: Kenan.Murphy@umassmed.edu
Keywords: Genetic Systems, DNA Replication, Microbial Pathogenesis

More on Kenan Murphy's Research Research | Figures | Publications | Rotations | Biography View All Sections on One Page