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Phase image of H9 colonies on MEF feeders show defined edges indicative of undifferentiated cells. Photo courtesy: Dr. Lisa Hall, Dept of Cell Biology, UMass Medical School | 
Colony of undifferentiated H1 human ES cells stained for markers SSEA-4 (green) and Oct-4 (red). Blue is DAPI stain that indicates DNA. Photo courtesy: John Butler, Dr. Jeanne Lawrence, Dept of Cell Biology, UMASS Medical School |
Alkaline Phosphatase (red) staining of undifferentiated H9 human ES cells. MEF feeders and ES cells at the colony periphery that are differentiating are not stained. DAPI detects DNA (blue). Photo courtesy: John Butler, Dr. Jeanne Lawrence, Dept of Cell Biology, UMASS Medical School | 
H9 human ES cells after 10 days differentiation show positive staining for nestin (green), a neural stem cell marker. Photo courtesy: John Butler, Dr. Jeanne Lawrence, Dept of Cell Biology, UMASS Medical School |
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H1 human ES cells induced to differentiate (neuronal) 6 days and stained for DAPI (DNA, blue) and pancytokeratin (green). Photo courtesy: John Butler, Dr. Jeanne Lawrence, Dept of Cell Biology, UMASS Medical School | .jpg "H9 rgb 216x165")
Hybridization for hnRNA on H9 human ES cell colony (red) MEF feeders are DAPI stained (blue). Photo courtesy: Dr. Lisa Hall, Dept of Cell Biology, UMass Medical School |
Undifferentiated H9 ES cells stained with DAPI (blue) and Tra-181 (red). Photo courtesy: John Butler, Dr. Jeanne Lawrence, Dept of Cell Biology, UMASS Medical School | .jpg "ssea3 216x165")
H9 ES cell colony stained for SSEA-3 (green). DAPI stains DNA in nuclei (blue). Photo courtesy: Dr. Prachi Ghule, Dr. Gary Stein, Dept of Cell Biology, UMASS Medical School |
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Synchronized S-phase H9 human ES cells stained for Cyclin E (red) and Ki67 (red). DAPI (blue) stains DNA. Photo courtesy: Dr. Prachi Ghule, Dr. Gary Stein, Dept of Cell Biology, UMASS Medical School | .jpg "Oct4 216x165")
H9 ES cell colony stained for Oct-4 (green). DAPI stains DNA in nuclei (blue). Photo courtesy: Dr. Prachi Ghule, Dr. Gary Stein, Dept of Cell Biology, UMASS Medical School |
Staining for Cyclin B1 (Red) and Ki67 (Green) in synchronized metaphase H9 ES cells. DNA stained with DAPI (blue)(100x). Photo courtesy: Dr. Prachi Ghule, Dr. Gary Stein, Dept of Cell Biology, UMASS Medical School | .jpg "Cyc dapi 216x165")
H9 ES cells stained for Cyclin A (green). DNA stained with DAPI (blue). Photo courtesy: Dr. Prachi Ghule, Dr. Gary Stein, Dept of Cell Biology, UMASS Medical School |
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H9 human ES cell colony shows cyclin B1 (green) staining of cells in late G2 and M phases of the cell cycle. DNA stained with DAPI (blue). Photo courtesy: Dr. Prachi Ghule, Dr. Gary Stein, Dept of Cell Biology, UMASS Medical School | 
Cyclin E (Red) and Ki67 (Green) in synchronized H9 ES cells. DAPI stains DNA in nuclei (blue). Photo courtesy: Dr. Prachi Ghule, Dr. Gary Stein, Dept of Cell Biology, UMASS Medical School |
H9 Embryoid body stained with Oct4 (red) and pancytokeratin (green). DNA stained with DAPI (blue). Photo courtesy: John Butler, Dr. Jeanne Lawrence, Dept of Cell Biology, UMASS Medical School | 
H9 Embryoid body stained with Oct4 (green) and pancytokeratin (red). DNA stained with DAPI (blue). Photo courtesy: John Butler, Dr. Jeanne Lawrence, Dept of Cell Biology, UMASS Medical School |
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