Contact Us

Co-Director:
Kahraman Tanriverdi, PhD
Phone: 508-856-6962
kahraman.tanriverdi@umassmed.edu
www.umassmed.edu/htcore

Possible Applications

High-Throughput Gene Expression Analysis

Ninety-six samples can be analyzed for 96 gene expression assays in under 3 hours. Standard TaqMan SNP assays or your own primers can be used. Users may also choose to submit their genes for DELTAgene assay design service provided by Fluidigm. Please contact us for more information.

High-Throughput miRNA Profiling

Ninety-six or more samples can be analyzed for any miRNA assay or entire miRNA assay library (1,801 miRNA Assays) in 1-5 business days. Qiagen miScript miRNA assays are provided by core.

High-Throughput miRNA Inhibitor & Mimic Transfection

Up to 96 miRNA inhibitor or mimic at a time can be transfected to desired cell line and up to 96 gene expression level can be analyzed in a few days with low cost. More than 1,200 miRNA inhibitors and mimics are available. Nucleofactor 96 shuttle system is also available for hard to transfect cells. 

SNP Genotyping

Ninety-six samples can be analyzed for 96 SNPs in under 3 hours. Standard TaqMan SNP assays can be used. Users may also choose to submit their SNPs for SNPtype assay design service provided by Fluidigm. Please contact us for more information.

High-Throughput Immunoassay Analysis

The lab can run up to 7 different ELISA assays from same set of samples at once robotically. This avoids freeze-thawing of samples as the assays can be run simultaneously by utilizing scheduling software integrated into the liquid handling robot software and a liquid sensing Varispan pipetting arm. Please email the lab with inquiries.

Single Cell Gene Expression Analysis

Ninety-six single cells can be analyzed for hundreds of gene expression assays. Single cells can be collected many ways such as flow cytometer cell sorting, micro injection or Laser micro dissection. RNA isolation is not required. Technique can be used small numbers of cells (up to 1,000 cells) too. Standard TaqMan SNP assays or your own primers can be used. Users may also choose to submit their genes for DELTAgene assay design service provided by Fluidigm. This service lowers the assay cost for gene expression analysis (approx. $155.00 for TaqMan vs. $30-75.00 for Fluidigm). Please contact with us for more detailed information.

Protein PCR Analysis - Proximity Extension Assay

Ninety-two proteins can be analyzed from 1 ┬Ál sample. Up to 96 samples can be analyzed paralel. This is possible through Fluidigm and Olink colloboration. Please visit http://www.olink.com/products/proseek-multiplex for more information or contact us.