Section: Rotations

Bert van den Berg, Ph.D.

Academic Role: Associate Professor

Faculty Appointment(s) In:
   Program in Molecular Medicine

Other Affiliation(s):
   Interdisciplinary Graduate Program



Rotation Projects

A number of rotation projects are available in the lab, giving students an opportunity to learn a variety of techniques, including recombinant DNA technology, growth of bacterial cultures, membrane protein purification, in vitro and in vivo transport assays, membrane protein crystallization, X-ray data collection, and membrane protein structure determination. Some specific rotation projects are:

OM transport of hydrophobic compounds:

1.  Cloning, expression, purification and crystallization of E. coli FadL mutants.

2. Development of assays to probe substrate specificity of E. coli FadL and other FadL family channels (e.g. hydrocarbon transporters).

OprD channels:

1. Cloning, expression, purification and crystallization of OprD channels from P. aeruginosa.

2. Assay development for determining OprD channel substrate specificity.

Other:

1. Cloning, expression and purification of eukaryotic metal transport proteins in the yeast Saccharomyces cerevisiae.

Office: Biotech 2, Suite 115
Phone: 508-856-1201
E-mail: Lambertus.VandenBerg@umassmed.edu
Keywords: Microbial Pathogenesis, Structural Biology, Biochemistry

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