Section: Rotations

Postdoctoral Position Available

Kirsten Hagstrom, Ph.D.

Academic Role: Assistant Professor

Faculty Appointment(s) In:
   Program in Molecular Medicine

Other Affiliation(s):
   Cell Dynamics Group
   Interdisciplinary Graduate Program

Potential Rotation Projects:

Novel chromosome segregation factors

A collection of proteins essential for proper chromosome segregation has been identified in a proteomics screen. These proteins will help us learn more about the fundamental mechanisms that ensure accurate cell division. Projects investigating these proteins could include any of the following:

• Characterize the RNAi depletion phenotype using time-lapse microscopy, antibody staining, and double RNAi combinations.
• Create a GFP-tagged transgene to follow protein dynamics in living cells.
• Immuno-localization in fixed cells and immunoprecipitation of associated proteins.
• Reiterative proteomics/RNAi to determine protein complexes and expand a protein interaction network.
• Isolate mutant alleles for genetic analysis.
• Perform biochemical assays as appropriate to suspected mechanism.

Condensin function

Condensin is conserved protein complex essential for shaping and separating eukaryotic chromosomes. Exploiting the advantages of the C. elegans system to probe condensin function will provide insights into general mechanisms of chromosome dynamics. Questions to address include:

• What proteins comprise condensin and how many different condensin complexes exist?
• How is condensin cell cycle regulated?
• How does condensin function during meiosis, and is there a distinct meiotic condensin complex?
• What is the role of condensin at the centromere?

Office: Suite 334
Phone: 508-856-6851
E-mail: Kirsten.Hagstrom@umassmed.edu
Keywords: Genetic Systems, Cancer, Cell Biology, Cell Cycle, Organisms - C. elegans

More on Kirsten Hagstrom's Research Research | Figures | Publications | Rotations | Biography View All Sections on One Page

Postdoctoral Position Available A Postdoctoral Position is available in Dr. Hagstrom's laboratory.  Contact her at the address above for additional details.