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Ravindra Singh, Ph.D.
Academic Role: Assistant Professor
Faculty Appointment(s) In:
Medicine
Other Affiliation(s):
Interdisciplinary Graduate Program
Molecular Genetics and Microbiology
Potential Rotation Projects
- Development of novel methods of in vivo SELEX and matrix-based
analysis protocols to understand the alternative splicing:
To understand the alternative splicing, a number of in vivo SELEX
experiments have been planned. This will lead to identification of novel
classes of exonic splicing enhancers (ESEs) and exonic splicing silencers
(ESSs). These results will be integrated to develop an advanced matrix-based
sequence analysis that will predict cis-elements.
- Identification of trans-acting factors that modulate alternative splicing:
Using in vivo SELEX, we have identified cis-elements
within exon 7 (of SMN pre-mRNA). Factors that bind to these cis-elements
will be identified by site-specific UV-crosslinking. Further, the RNA-protein
interactions will be probed using in vitro splicing and RNA binding
assays.
- Genomic SELEX to identify novel RNA-protein interactions:
The nucleic acid binding property of SMN and SMN complexes
will be studied using powerful techniques of genomic SELEX. The obtained results
will form the basis for chip based (DNA and protein microarrays) analysis of
SMN-associated factors (or transcripts) that are differentially expressed in
different cell lines. The outcome of this project will have diagnostic
application for diseases related to SMN gene(s).
- Aptamer-mediated modulation of splicing:
As an extension to our in vivo SELEX experiments, we will use selected
sequences (aptamers) in trans to modulate splicing of SMN gene.
The outcome of this project will have direct therapeutic application.
Office: Research 326
Phone: 508-856-1333
Fax: 508-856-6797
E-mail: Ravindra.Singh.Forward@umassmed.edu
Keywords:
RNA Biology,
Gene Therapy,
RNA Splicing,
Gene Expression,
Gene Regulation
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55 Lake Avenue North Worcester, MA 01655