The Flow Cytometry Core Facility offer a wide variety of training opportunities
Our FACSAria self-sorting training program: Please drop by the main lab (S5-322) or call the core at (6-3276) if you are interested in this advanced training opportunity. LSR operator experience is a pre-requisite. Candidates should already be experienced with the FACSDiva software and the principles of flow cytometry.The program is intended for laboratories and individuals who have a need to sort regularly after normal core facility hours, 8 AM – 5 PM Monday-Friday. Training involves one 3 hr class, followed by two scheduled sorts that you perform with staff assistance. Permission to self-sort after hours is then granted at the discretion of the staff. Sort training is billed as sorting time and is subject to our cancellation policy. Cancellations within 24 hr will be subject to a 2 hr charge.
The Sony SH800 sorter is more automated, and training begins by observing a scheduled staff-assisted sort, followed by one sort that you schedule and perform during staff hours.
The FACSCalibur training session is a great introduction to flow cytometry. This 1 hour course covers the basics and allows you to operate our self-use Caliburs located in the main facility. These instruments can analyse up to 4 colors (plus light scatter) and operate with Cellquest Macintosh software. Help is always available after the training to get you started performing your own analysis at a reduced hourly rate.
A 3 hour LSR II training course is required for those who wish to operate the self use instruments in the LRB room 357 and the LSRIIs located in the Flow Core S5-322. Training charges are per person at our normal training rate. This course covers the operation, maintenence, data handling, FACSDiva software and other practical aspects of using a multi-laser analysis instrument. The course is offered on the first Monday and third Thursday of each month with a maximum of 5 students per class. Space permitting, you can attend for a refresher without charge once the course has been taken. If there is sufficient demand additional courses may be scheduled each month if the core facility staffing allows. Please contact Ted to schedule your training.
Advanced LSR II training is available which focuses on how the instrument and FACSDiva software can best be utilized. Depending on your needs this can cover 1) fluorochrome selection and custom filter configuration for multi-color analysis, 2) compensation theory and use of the auto-comp feature in FACSDiva, 3) cell cycle and other DNA applications, 4) calcium flux to name a few examples. These sessions can be individualized to your specific application. Please call the core facility at 508-856-3276 and we can schedule a session with a specialist suited to your field of research.
Flowjo is a powerful off-line flow cytometry analysis software package offered at a signifcant discount due to our UMMS site license. Excellent tutorials can be found at FlowJo.com and we offer introductory training to familiarize you with this popular application. UMass staff can join the subscription by going to Flowjo.com and downloading the most recent version for your operating system. Then go to flowjo.com/FLS/registration/umass.html to register and get your serial number to activate your software. Charges for FlowJo are approximately $55/quarter (based on our current client number) beginning May 1 of each year. To avoid charges for unused licenses, the subscription must be cancelled - to cancel, please contact Dr. Rachel Gerstein. Dr. Gerstein will also train users in FlowJo by request and can be consulted about data analysis strategies
When scheduling an analysis with a facility operator the investigator is welcome to participate in the set-up of your experiment. These can be very useful and informative collaborations! We need your participation for cell sorting appointments in order to set the gates for your population(s) of interest.