General Sorting Information

 

Machine

Core Lab Aria

BSL3 Aria

 MoFlo

 DV-1

Lasers

488nm, 633nm, 405nm, 350nm, 561nm

 488nm, 633nm, 405nm, 350nm

488nm, 633nm,
405nm

488nm, 633nm,
350nm

 Nozzle size
(other option)

70 um 85 & 100um

70um 100um

 70um
100um

 80um
70um

 Sort Tubes

polystyrene or polyproylene

polystyrene or polypropylene

 polypropylene
BD 352063, 352002

 polystyrene
BD 352058, 352008

 Cell Concentration ***

 1-2 x107

 1-2 x107

 1-2 x107

 1-2 x107

 Sort Rate ***

10,000 - 30,000 cells/second

 10,000 - 30,000 cells/second

10,000 - 30,000 cells/second

5,000 - 12,000 cells/second

Operating Pressure70 psi 45 psi 20psi
 70psi 20 psi

 60psi  30psi

 30psi


Running media - 1% FBS in any media.  HEPES can be added at a final concentration of 25mM to media to keep cells well buffered for all day sorts, and/or to collection media.

Collection media - this is usually 20% FBS in whichever media the cells are happiest.  This is diluted 4-5x and ends up being approximately 5% FBS. 

Filtering :

All samples are filtered through 40 µm mesh prior to running by core facility personel, however if you wish to filter the samples yourself refer to the ordering information below.

Sterile 12x75 mm tubes with filter caps are available; Becton Dickinson Cat. number 352235 or Fisher Cat. number 08-771-23       

Nitex Nylon Filtration Mesh order from:    Small Parts

                   (800-220-4242) x403 Elvin

                   (for bulk orders with a minimum of 3 yards) Nitex 03-41/31

                    12" x 12" pieces, 6 per pack CMN-LP041031-06 

***  All numbers are an average and actual numbers will vary based on cell type and size and/or sorting options.  ***