Instrumentation

Amnis Flowsight Imaging Cytometer

Flowsight Imaging Channel Guide

This unique cytometer quantitatively detects brightfield, darkfield and fluorescent images with high sensitivity CCD cameras. The fluorescence amplification and minimal noise detection results in very high signal sensitivity. In addition to being able to see the cells displayed in dot plots and histograms, the imaging provides analysis tools not available in conventional cytometers such as texture and spot counting. In the photo above, the color dot plot is a 5-way differential of cells in whole blood using only CD45 fluorescence and darkfield side-scatter. The Flowsight is equipped with violet, blue and red lasers for fluorescence detection in up to 10 channels. Running this instrument is straightforward requiring only laser power adjustment. Make an appointment and core facility personnel will run your samples while showing you how to use the instrument independently.

BD FACSCaliburs


FACSCalibur Fluorochome Selection Guide

These are dual-beam (air-cooled lasers) analyzers similar to the FACScan in capabilities with the addition of the second laser for four color immunofluorescence analysis. The second laser is a red-emitting diode, with output at 635nm only, suitable for excitation of APC (Allophycocyanin) or Alexa 647. Aside from the dedicated FACSCalibur for core facility analysis, we also have a second instrument for investigator performed analysis (after completing our training course, or demonstrating proficiency).

BD LSR IIs

LRB Satellite lab

LRB LSR A "Uncle  Fester" Fluorochome Selection Guide

LRB LSR B :"Lurch" Fluorochome Selection Guide

Core Facility A-5 laser: "Donald" LSR Fluorochrome Selection Guide

Core Facility B-3 Laser LSR: "Daisy" Fluorochome Selection Guide

The LSR II is a dital multiparameter flow cytometer analyzer. It features the Digital DIVA hardware and software. There are 2 LSR II's housed in the LRB; LSR A "UNcle Fester" has 4 lasers and 10 fluorescent detectors and LSR B "Lurch" has 2 lasers and 8 fluorescent detectors. These LSR II's are primarily for investigator performed analysis and can be used after completing training from the core facility. The core facility LSR II B-3 "Daisy" has 3 lasers and 9 fluorescent detectors and is also available for investigator preformed analysis after training. The core facility LSR II A-5 "Donald" has 5 lasers and 18 fluorescent detectors and is reserved for core facility analysis.  Due to their more complex nature, please schedule LSR II experiments that you would like us to run well in advance with any staff member (6-3276).

Beckman Coulter MoFlo XDP Cell Sorter

MoFlo XDP Fluorochome Selection Guide

The three laser MoFlo XDP digital cell sorter is configured with 1 color on the 633nm laser, 1 on the 405nm laser, and 5 colors on the 488nm laser. This machine is ideal for sorting large numbers of small cells.

BD FACSVantage DV-1 Cell Sorter

FACSVantage Fluorochome Selection Guide

The configuration is 5 fluorescent detectors from the 488nm laser, 2 detectors from the UV laser and 1 detector (APC) from the red laser utilizing the octagons and trigon detectors found in the BD FACSAria, LSRII and FACSCantos.

Core Lab BD FACSAria II Cell Sorter

Core Lab FACSAria II Fluorochome Selection Guide

The Core Lab Aria is configured with 5 parameters on the 488nm laser, 3 parameters of the 633nm laser, 3parameters on the 405nm laser, 3 parameters on the 350nm laser, and five parameters on the 561nm laser. With this machine, we are now able to sort 561nm excited fluorochromes such as mCherry. The 561 nm laser is also ideal for sorting RFP. This sorter is available for self-sorting after hours (after 5 pm) for investigators who have completed the training session described on our training page.

BSL3 BD FACSAria Cell Sorter

BSL3 FACSAria Fluorochome Selection Guide

The BSL3 Aria is configured with 5 parameters on the 488nm laser, 3 parameters on the 633nm laser, 3 parameters on the 405nm laser, and 3 parameters on the 350nm laser. This machine is housed in a BSL3 facility allowing us to sort unscreened human and/or infectious samples up to biosafety level 3.

When scheduling cell sorting please refer to the guides below.

General Sorting Information

What to bring to a sort