RNAi Core Facility

UMMS has purchased the complete collections of human and mouse retroviral and lentiviral short hairpin RNA (shRNA) libraries from Open Biosystems, and has negotiated with them to distribute clones to UMass researchers (Amherst, Boston, Dartmouth, Lowell and Worcester campuses ONLY) at a reduced price.

The UMMS RNAi Core Facility currently has the following genome-wide libraries available for distribution: 

  • Human and mouse retroviral (pSM2) shRNAmir libraries
    • Human releases 1.3-1.20 (~90,000 constructs)
    • Mouse releases 2.3-2.17 (~70,000 constructs)
    • Control clones (non-silencing, eGFP and luciferase)
  • Human and mouse lentiviral (pGIPZ) shRNAmir libraries
    • Human releases 6.1 to 6.15 (~70,000 constructs)
    • Mouse releases 7.1 to 7.12 (~60,000 constructs)
    • Control clones (non-silencing, GAPDH and empty vector)
  • Human and mouse lentiviral (TRC) shRNA libraries
    • Human (~85,000 constructs)
    • Mouse (~79,000 constructs)
    • Control clones (empty vector and eGFP)

Complete information regarding the retroviral and lentiviral shRNAmir libraries and the lentiviral TRC libraries can be found on the Open Biosystems website. In addition, please refer to the  Nature Methods paper and Nature Genetics Technical Report for information on the libraries.