Protocols
Antibody DNA-Hybridization
Antibody RNA-Hybridization
Culture and Fixation of Cells
DNA Hybridization
Nick translation
RNA DNA Hybridization
RNA Hybridization
Additional Resources
More in depth discussion of the development and applications of DNA/RNA in
situ hybridization methodology can be found in the following articles (PDFs):
Tam, R., Shopland, L., Johnson, C.V., McNeil, J. and Lawrence, J.B, 2002. “Applications of RNA FISH for visualizing gene expression and nuclear architecture”, in the book series, FISH: A Practical Approach, Oxford University Press.
Johnson, C. V., and Lawrence, J. B. (1991). Genetic Analysis Techniques and Applications, 8, 75.
McNeil, J. A., Johnson, C. V., Carter, K. C., Singer, R. H., and Lawrence, J. B. (1991). Genetic Analysis Techniques and Applications, 8, 41.
Lawrence, J. B. 1990. A fluorescence in situ hybridization approach for gene mapping and the study of nuclear organization, p. 1. In Editor (ed.), Genome Analysis Volume 1: Genetic and Physical Mapping, Cold Spring Harbor Press, Cold Spring Harbor NY.