Section: Figures

Postdoctoral Position Available

Lab Page Link

Greenfield Sluder, Ph.D.

Academic Role: Professor

Faculty Appointment(s) In:
   Cell Biology

Other Affiliation(s):
   Cell Dynamics Group
   Interdisciplinary Graduate Program

Figures

Figure 1:   Mammalian cells. (A and B) Overlaid phase and fluorescence images showing BrdU incorporation in mononucleate and binucleate cells. (A) Cells were previously treated with 0.5 µM cytochalasin D and cultured on bare glass. Mononucleate cells have incorporated BrdU, whereas the binucleates have not. (B) Cells treated with 0.5 µM cytochalasin D and cultured on fibronectin-coated glass. Both the mononucleate and binucleate cells have incorporated BrdU. (C) Cells previously treated with 0.5 µM cytochalasin D and cultured on fibronectin-coated glass (images taken from Video 4, available at http://www.jcb.org/cgi/content/full/jcb.200403014/DC1). Frames from a video sequence of two binucleate cells in the same field progressing through mitosis. The first to enter mitosis (top row) divides into two, whereas the second (bottom row) divides into three. Phase-contrast microscopy. Times are in h:min after cytochalasin D removal. Bars, 50 µm.

Figure 2:  Repeated rounds of centrosome duplication in an aphidicolin-treated Xenopus egg extract.  Frames from a time-lapse video sequence, showing the increase in aster number over time in a microscope field.  The decrease in aster number in panel d is due to the migration of asters from the plane of focus and field of view.  Minutes after addition of sperm nuclei are seen in the lower right corner of each frame.  Polarization optics. 10 microns per scale division.

In other studies we have characterized the cell cycle checkpoint controls for the metaphase-anaphase transition. These checkpoint pathways serve to ensure the equal distribution of chromosomes during cell division. We have demonstrated that signal transducing molecules in the kinetochore monitor chromosome attachment to the spindle and that even one unattached kinetochore will block the metaphase-anaphase transition.

Using GFP-cyclin B and confocal microscopy we are also investigating how the checkpoint pathway that monitors the completion of DNA synthesis controls nuclear envelope breakdown and entry of the cell into mitosis.

Office: Biotech IV, Suite 324
Phone: 508-856-8651
E-mail: Greenfield.Sluder@umassmed.edu
Keywords: Biophysics, Cell Biology, Cell Cycle

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Postdoctoral Position Available A postdoctoral position is available to study in this laboratory. Contact Dr. Sluder for additional details.